Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)

Novus Biologicals | Catalog # NBP2-00537

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3D8
View all Nicotinamide N-Methyltransferase/NNMT Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NNMT(NP_006160) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells and NNMT-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-NNMT monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Immunocytochemistry/ Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunocytochemistry/ Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunocytochemistry/Immunofluorescence: NNMT Antibody (3D8) [NBP2-00537] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT.
Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human prostate tissue using anti-NNMT mouse monoclonal antibody.
Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537] - Analysis of Jurkat cells, using anti-NNMT antibody (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Western Blot: NNMT Antibody (3D8) [NBP2-00537] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.
Western Blot: NNMT Antibody (3D8) [NBP2-00537] Analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody.
Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NNMT mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NNMT mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human endometrium tissue using anti-NNMT mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human liver tissue using anti-NNMT mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537]

Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human pancreas tissue using anti-NNMT mouse monoclonal antibody.
Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)

Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)[NBP2-00537]

Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)[NBP2-00537]Simple Western� analysis of endogenous protein CDK2 from HELA lysates (0.2 mg/mL) using CDK2 Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nicotinamide N-Methyltransferase/NNMT

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq].

Long Name

Nicotinamide N-Methyltransferase

Alternate Names

Nicotinamide NMethyltransferase, NNMT

Entrez Gene IDs

4837 (Human)

Gene Symbol

NNMT

Additional Nicotinamide N-Methyltransferase/NNMT Products

Product Documents for Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)

Certificate of Analysis

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Product Specific Notices for Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)

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Protocols

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