Niemann-Pick type C2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84012

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Niemann-Pick Type C2/NPC2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIVSH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Niemann-Pick type C2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012]

Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012]

Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012]

Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012]

Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012]

Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012]

Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in Leydig cells and cells in seminiferous ducts.
Niemann-Pick type C2 Antibody - BSA Free Western Blot: Niemann-Pick type C2 Antibody - BSA Free [NBP1-84012]

Western Blot: Niemann-Pick type C2 Antibody - BSA Free [NBP1-84012]

Analysis in human cell lines U2OS and HeLa using Anti-NPC2 antibody. Corresponding NPC2 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
Niemann-Pick type C2 Antibody - BSA Free Western Blot: Niemann-Pick type C2 Antibody - BSA Free [NBP1-84012]

Western Blot: Niemann-Pick type C2 Antibody - BSA Free [NBP1-84012]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Niemann-Pick type C2 Antibody - BSA Free Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody - BSA Free [NBP1-84012]

Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody - BSA Free [NBP1-84012]

Analysis in human epididymis and skeletal muscle tissues using NBP1-84012 antibody. Corresponding NPC2 RNA-seq data are presented for the same tissues.
Niemann-Pick type C2 Antibody - BSA Free Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody - BSA Free [NBP1-84012]

Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody - BSA Free [NBP1-84012]

Staining of human epididymis shows strong granular cytoplasmic positivity in glandular cells).

Applications for Niemann-Pick type C2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 1 using NBP1-84012 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Niemann-Pick Type C2/NPC2

Niemann-Pick type C2 encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy. [provided by RefSeq]

Alternate Names

EDDM1, Epididymal Protein 1, HE1, NiemannPick Type C2, NPC2

Gene Symbol

NPC2

Additional Niemann-Pick Type C2/NPC2 Products

Product Documents for Niemann-Pick type C2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Niemann-Pick type C2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Niemann-Pick type C2 Antibody - BSA Free

Customer Reviews for Niemann-Pick type C2 Antibody - BSA Free (1)

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Customer Images

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  • Niemann-Pick type C2 Antibody
    Name: jian li
    Application: Immunocytochemistry
    Sample Tested: hela cell
    Species: Human
    Verified Customer | Posted 06/14/2017
    IF for LAMP1 and NPC2
    for LAMP1 staining, mouse anti LAMP1 antibody was used followed by AF488 anti mouse secondary. For NPC2 stating, rabbit anti NPC2 was used with 1:100 dilution followed by AF568 anti rabbit secondary. shown is a merged image of LAMP1 (GREEN) and NPC2 (RED)
    Niemann-Pick type C2 Antibody - BSA Free NBP1-84012

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Protocols

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FAQs for Niemann-Pick type C2 Antibody - BSA Free

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  • Q: I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?

    A:

    Niemann-Pick C1 Antibody (NB400-148) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # H00010577-D01PInnovators Reward Program.

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