NIF1 Antibody

Western Blot: NIF1 Antibody [NB100-2579]

Western Blot: NIF1 Antibody [NB100-2579] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. NB100-2579 used for WB at 0.04 ug/ml (A) and at 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). NIF1 was also immunoprecipitated by rabbit anti-NIF1 antibody BL2737 (NB100-2579) and BL2738. Detection was by chemiluminescence, with exposure times of 3 minutes (A) and 30 seconds (B).

Immunohistochemistry-Paraffin: NIF1 Antibody [NB100-2579]

Immunohistochemistry-Paraffin: NIF1 Antibody [NB100-2579] - Section of human Hodgkin's lymphoma. Antibody: Affinity purified rabbit anti- NIF1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Flow Cytometry: NIF1 Antibody [NB100-2579] -

Zfp335 is involved in T cell homeostasis A Schematic diagram of T cell adaptive transfer assay. Naive CD4 + T cells (5 × 106) and CD8 + T cells (2 × 106) from WT (ERCreCD45.1 + ) and KO (ERCreZfp335fl/flCD45.2 + ) mice pretreated with Tamoxifen were stained with CTV, and then were mixed at a 1:1 radio and intravenously co-transferred into sublethally irradiated (4.5 Gy) recipient mice (CD45.1 + CD45.2 + ). On day 7 after adoptive transfer, cells from spleens and lymph nodes of the recipients were performed FACS analysis. B ZFP335 expression in naive T cells (CD4 + CD25-CD44-CD62L +, CD8 + CD44-CD62L + ) of WT mice, and in the transferred T cells (CD45.1 + CD4 + /CD8 + ) of sub-lethally irradiated (4.5 Gy) WT mice. C and D MFI statistics of ZFP335 in CD4 + T cells (C) and CD8 + T cells (D). E Representative FACS plots of donor CD4 + T cells and CD8 + T cells (WT donor: CD45.1 + CD4 + /CD8 + ; KO donor: CD45.2 + CD4 + /CD8 + ) in the recipient's spleen and lymph nodes. F–I Statistical frequencies of WT (CD45.1 + ) and KO (CD45.2 + ) T cells in spleens and lymph nodes of the recipient mice (n = 8). Data are representative of three independent experiments shown as the mean +/- s.e.m. Statistical significance was determined by two-sided unpaired t tests. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41088342), licensed under a CC-BY license. Not internally tested by Novus Biologicals.