NIPP1 Antibody
Novus Biologicals | Catalog # NB100-1286
![Western Blot: NIPP1 Antibody [NB100-1286]](https://beta-resources.rndsystems.com/images/products/NIPP1-Antibody-Western-Blot-NB100-1286-img0006.jpg "Western Blot: NIPP1 Antibody [NB100-1286]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-EAWPGKKPTPSLLI corresponding to C-Terminus according to NP_002704.1, NP_054829.2, NP_612568.1.
Specificity
This antibody is expected to recognise all three human isoforms of this protein.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Novus Biologicals Goat NIPP1 Antibody (NB100-1286) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-NIPP1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for NIPP1 Antibody
Western Blot: NIPP1 Antibody [NB100-1286]
Western Blot: NIPP1 Antibody [NB100-1286] - (1ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and NIH3T3 (D) nuclear lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.![Immunohistochemistry-Paraffin: NIPP1 Antibody [NB100-1286]](https://beta-resources.rndsystems.com/images/products/NIPP1-Antibody-Immunohistochemistry-Paraffin-NB100-1286-img0005.jpg "Immunohistochemistry-Paraffin: NIPP1 Antibody [NB100-1286]")
Immunohistochemistry-Paraffin: NIPP1 Antibody [NB100-1286]
Immunohistochemistry-Paraffin: NIPP1 Antibody [NB100-1286] - 3.8ug/ml staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for NIPP1 Antibody
Application
Recommended Usage
Immunohistochemistry
2.5 - 3.8 ug/ml
Immunohistochemistry-Paraffin
2.5 - 3.8 ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
1 - 2 ug/ml
Application Notes
Western blot: Approx 40kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Lung for this particular batch.
IHC: In paraffin embedded Human Thymus shows speckled staining of nuclei.
IHC: In paraffin embedded Human Thymus shows speckled staining of nuclei.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NIPP1
Long Name
Nuclear Inhibitor of Protein Phosphatase 1
Alternate Names
ARD1, PPP1R8, PRO2047, RNase E
Gene Symbol
PPP1R8
UniProt
Additional NIPP1 Products
Product Documents for NIPP1 Antibody
Product Specific Notices for NIPP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for NIPP1 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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