NKAP Antibody

Novus Biologicals | Catalog # NBP3-48584

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NKAP. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NKAP Antibody

NKAP Antibody

Immunohistochemistry: NKAP Antibody [NBP3-48584] -

NKAP antibody detects NKAP protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
NKAP antibody (NBP3-48584) dilution: 1:500.


Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
NKAP Antibody

Western Blot: NKAP Antibody [NBP3-48584] -

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NKAP antibody (NBP3-48584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NKAP Antibody

Western Blot: NKAP Antibody [NBP3-48584] -

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NKAP antibody (NBP3-48584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NKAP Antibody

Immunohistochemistry: NKAP Antibody [NBP3-48584] -

NKAP antibody detects NKAP protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
NKAP antibody (NBP3-48584) dilution: 1:500.


Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
NKAP Antibody

Western Blot: NKAP Antibody [NBP3-48584] -

NKAP antibody detects NKAP protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NKAP antibody (NBP3-48584) at a dilution of 1:1000.

Applications for NKAP Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKAP

NKAP is a transcriptional repressor that associates with the NOTCH (see MIM 190198) corepressor complex and is requiredfor T-cell development (Pajerowski et al., 2009 (PubMed 19409814)).(supplied by OMIM)

Alternate Names

FLJ22626, NF-kappaB activating protein, NF-kappa-B-activating protein, NFKB activating protein

Gene Symbol

NKAP

Additional NKAP Products

Product Documents for NKAP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NKAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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