NKCC2/SLC12A1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80993

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NKCC2/SLC12A1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NKCC2/SLC12A1 Antibody - BSA Free

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Analysis in human kidney and pancreas tissues using NBP1-80993 antibody. Corresponding SLC12A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human cerebellum, kidney, liver and pancreas using Anti-SLC12A1 antibody NBP1-80993 (A) shows similar protein distribution across tissues to independent antibody NBP1-82559 (B).
Western Blot: NKCC2/SLC12A1 Antibody [NBP1-80993]

Western Blot: NKCC2/SLC12A1 Antibody [NBP1-80993]

NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-80993-img0011.jpg
Immunocytochemistry/ Immunofluorescence: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunocytochemistry/ Immunofluorescence: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunocytochemistry/Immunofluorescence: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human cell line RPTEC TERT1 shows localization to nucleus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.
Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human kidney shows strong membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for NKCC2/SLC12A1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKCC2/SLC12A1

The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. [provided by RefSeq]

Long Name

Sodium/Potassium/Chloride Transporter NKCC2

Alternate Names

BSC1, SLC12A1

Entrez Gene IDs

6557 (Human)

Gene Symbol

SLC12A1

UniProt

Q13621 (Human)

Additional NKCC2/SLC12A1 Products

Product Documents for NKCC2/SLC12A1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NKCC2/SLC12A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NKCC2/SLC12A1 Antibody - BSA Free

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Protocols

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