NKX2.2 Antibody (3E4) - BSA Free

Novus Biologicals | Catalog # NBP2-37486

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3E4

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human NKX2.2 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NKX2.2 Antibody (3E4) - BSA Free

Western Blot: NKX2.2 Antibody (3E4)BSA Free [NBP2-37486]

Western Blot: NKX2.2 Antibody (3E4)BSA Free [NBP2-37486]

Western Blot: NKX2.2 Antibody (3E4) [NBP2-37486] - Western blot analysis using NKX2.2 mAb against human NKX2.2 recombinant protein.(Expected MW is 22 kDa)
Flow Cytometry: NKX2.2 Antibody (3E4) - BSA Free [NBP2-37486]

Flow Cytometry: NKX2.2 Antibody (3E4) - BSA Free [NBP2-37486]

Flow Cytometry: NKX2.2 Antibody (3E4) [NBP2-37486] - Flow cytometric analysis of MCF-7 cells using NKX2.2 mouse mAb (green) and negative control (red).
ELISA: NKX2.2 Antibody (3E4) - BSA Free [NBP2-37486]

ELISA: NKX2.2 Antibody (3E4) - BSA Free [NBP2-37486]

ELISA: NKX2.2 Antibody (3E4) [NBP2-37486] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for NKX2.2 Antibody (3E4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKX2.2

Nkx2.2, or Homeobox protein Nkx-2.2, consists of a 273 amino acid isoform that is 30 kDa, and is involved in controlling and maintaining the expression of genes within pancreatic endocrine cells and axons. Current research is being conducted on the relationship between Nkx2.2 and a variety of diseases and disorders, including glioma, neuronitis, maturity-onset diabetes, pancreatitis, Partington syndrome, sarcoma, gastrointestinal neuroendocrine tumor, acute lymphoblastic leukemia, multiple sclerosis, and carcinoid tumors. The protein has been linked to the Wnt, Hedgehog, and notch pathways, as well as regulation of beta-cell development and gene expression. NKx2.2 interacts with several other proteins, including OLIG2, HDAC1, SIN3A, FOXA2, and FOXO1.

Long Name

NK2 Homeobox 2

Alternate Names

NKX2B

Gene Symbol

NKX2-2

Additional NKX2.2 Products

Product Documents for NKX2.2 Antibody (3E4) - BSA Free

Certificate of Analysis

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Product Specific Notices for NKX2.2 Antibody (3E4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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