NLRP3/NALP3 Antibody

Novus Biologicals | Catalog # NB100-41104

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NLRP3/NALP3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DLYEKAKRDEPK corresponding to internal region of NLRP3/NALP3 Antibody according to NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1.

Specificity

This antibody is expected to recognise all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NLRP3/NALP3 Antibody

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NB100-41104]

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NB100-41104]

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NB100-41104] - Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts.
NLRP3/NALP3 Antibody

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody [NB100-41104] -

Activation of NLRP3 inflammasome in platelets from patients with active Crohn’s disease (CD). (A) Co-immunoprecipitation of ASC with NLRP3 in platelets from the healthy group and patients with active CD (i). Quantification of the interaction between ASC and NLRP3 in platelets was below (n = 3), (ii). (B) Immunofluorescence staining showed colocalization of ASC (green) and NLRP3 (red) in platelets from patients with active CD (i). The experiment was repeated three times. Intensity traces were plotted below (ii). Scale bars were 20 μm. Health, the healthy group; CD, the patients with active Crohn’s disease. Data are presented as mean +/- SD, ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34262463), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for NLRP3/NALP3 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2 - 4 ug/ml

Peptide ELISA

Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave an approx. 70 kDa band in human lymph node and tonsil lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 118 kDa band according to NP_004886.3 and 106 kDa band according to NP_899632.1. The 70 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NLRP3/NALP3

NACHT, LRR and PYD domains-containing protein 3 (NALP3), Nucleotide-Binding Oligomerization Domain, or Leucine Rich Repeat and Pyrin Domain Containing 3 (NLRP3) (human NLRP3-isoform 2 theoretical molecular weight 118kDa) acts as a cytosolic receptor for stress-signals initiated by a wide variety of pathogen-associated molecular patterns (PAMPS) and danger-associated molecular patterns (DAMPs) (1). NLRP3 belongs to the family of NOD-like receptors, a type of pattern-recognition receptor (PRR) which participates in innate immune responses. The exact mechanisms leading to NLRP3 activation are still not fully resolved. Some proposed mechanisms for NLRP3 inflammasome activation include induced ion channel flux as exemplified by potassium efflux, sodium influx and calcium signaling, generation of mitochondrial reactive oxygen species (ROS), and lysosomal destabilization (2). Activation of NLRP3 induces its association with the adaptor protein Apoptosis-associated Speck-like protein containing CARD (ASC) forming a complex that binds to Caspase-1 (1, 2). The inflammasome complex, comprised of NLRP3, ASC and Caspase 1, induces the processing of pro-IL-1beta and pro-IL-18 and release of the mature inflammatory cytokines IL-1beta and IL-18 and pyroptosis (1-3).

NLRP3 is expressed in a variety of cell types such as monocytes, dendritic cells, lymphocytes and epithelial cells (3). Abnormal NLRP3 activation may occur as the result of inherited mutations and is associated with diseases such as hereditary periodic fevers (HPFs) and familial cold autoinflammatory syndrome (FCAS). Additionally, abnormal NLRP3 activation is associated with a variety of diseases such as gout, obesity, atherosclerosis, Alzheimers, multiple sclerosis and type 2 diabetes (1, 3). NLRP3 inflammasome is regulated by several post-translational modifications (e.g., ubiquitination, phosphorylation and sumoylation) as well as by various interacting proteins (e.g., JNK1, FBXO3, TXNIP, MARK4 and PKD) (4).

References

1. Abderrazak, A., Syrovets, T., Couchie, D., El Hadri, K., Friguet, B., Simmet, T., & Rouis, M. (2015). NLRP3 inflammasome: From a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases. Redox Biology. https://doi.org/10.1016/j.redox.2015.01.008

2. Yang, Y., Wang, H., Kouadir, M., Song, H., & Shi, F. (2019). Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors. Cell Death and Disease. https://doi.org/10.1038/s41419-019-1413-8

3. Zahid, A., Li, B., Kombe, A. J. K., Jin, T., & Tao, J. (2019). Pharmacological inhibitors of the nlrp3 inflammasome. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02538

4. Kelley, N., Jeltema, D., Duan, Y., & He, Y. (2019). The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms20133328

Long Name

NLR Family, Pyrin Domain Containing 2

Alternate Names

AGTAVPRL, AII, CIAS1, Cryopyrin, FCAS, FCU, MWS, NACHT, NALP3, PYPAF1

Entrez Gene IDs

114548 (Human)

Gene Symbol

NLRP3

UniProt

NP_004886.3

Additional NLRP3/NALP3 Products

Product Documents for NLRP3/NALP3 Antibody

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Product Specific Notices for NLRP3/NALP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NLRP3/NALP3 Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for NLRP3/NALP3 Antibody

Showing  1 - 4 of 4 FAQs Showing All

  • Q: Are the NLRP3/NALP3 antibodies validated in Simple Western?

    A: Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.

  • Q: Does NLRP3/NALP3 antibodies come in lyophilized form?

    A: Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.

  • Q: What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

    A: A sequence within the internal region (C-DLYEKAKRDEPK) of NLRP3/NALP3.

  • Q: What is the theoretical molecular weight for NLRP3/NALP3 antibodies?

    A: The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.

  • Q: Are the NLRP3/NALP3 antibodies validated in Simple Western?

    A: Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.

  • Q: Does NLRP3/NALP3 antibodies come in lyophilized form?

    A: Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.

  • Q: What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

    A: A sequence within the internal region (C-DLYEKAKRDEPK) of NLRP3/NALP3.

  • Q: What is the theoretical molecular weight for NLRP3/NALP3 antibodies?

    A: The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.

  • Q: Are the NLRP3/NALP3 antibodies validated in Simple Western?

    A: Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.

  • Q: Does NLRP3/NALP3 antibodies come in lyophilized form?

    A: Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.

  • Q: What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

    A: A sequence within the internal region (C-DLYEKAKRDEPK) of NLRP3/NALP3.

  • Q: What is the theoretical molecular weight for NLRP3/NALP3 antibodies?

    A: The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.

  • Q: Are the NLRP3/NALP3 antibodies validated in Simple Western?

    A: Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.

  • Q: Does NLRP3/NALP3 antibodies come in lyophilized form?

    A: Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.

  • Q: What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

    A: A sequence within the internal region (C-DLYEKAKRDEPK) of NLRP3/NALP3.

  • Q: What is the theoretical molecular weight for NLRP3/NALP3 antibodies?

    A: The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.

Showing  1 - 4 of 4 FAQs Showing All
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