NM23-H2/NME2 Antibody (2E3) - BSA Free
Novus Biologicals | Catalog # NBP2-60241
![Western Blot: NM23-H2/NME2 Antibody (2E3)BSA Free [NBP2-60241]](https://resources.rndsystems.com/images/products/NM23-H2-NME2-Antibody-2E3-Western-Blot-NBP2-60241-img0001.jpg "Western Blot: NM23-H2/NME2 Antibody (2E3)BSA Free [NBP2-60241]")
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2E3
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human NM23-H2/NME2 (1-152aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for NM23-H2/NME2 Antibody (2E3) - BSA Free
Western Blot: NM23-H2/NME2 Antibody (2E3)BSA Free [NBP2-60241]
Western Blot: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H2/NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: A549 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: LnCap cell lysateThe cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: NME2 transfected 293T cell lysate![Immunocytochemistry/ Immunofluorescence: NM23-H2/NME2 Antibody (2E3) - BSA Free [NBP2-60241]](https://resources.rndsystems.com/images/products/NM23-H2-NME2-Antibody-2E3-Immunocytochemistry-Immunofluorescence-NBP2-60241-img0002.jpg "Immunocytochemistry/ Immunofluorescence: NM23-H2/NME2 Antibody (2E3) - BSA Free [NBP2-60241]")
Immunocytochemistry/ Immunofluorescence: NM23-H2/NME2 Antibody (2E3) - BSA Free [NBP2-60241]
Immunocytochemistry/Immunofluorescence: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Applications for NM23-H2/NME2 Antibody (2E3) - BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NM23-H2
Long Name
Non-metastatic Protein 23 Homolog 2
Alternate Names
NDK B, NDP Kinase B2, NDPKB, NM23B, NME2
Gene Symbol
NME2
Additional NM23-H2 Products
Product Documents for NM23-H2/NME2 Antibody (2E3) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NM23-H2/NME2 Antibody (2E3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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