NMDAR2A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19551

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NMDAR2A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human NMDAR2A. The exact sequence is proprietary.

Localization

Cell membrane; Multi-pass membrane protein; Cell junction; synapse; postsynaptic cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

165 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NMDAR2A Antibody - BSA Free

Immunohistochemistry-Paraffin: NMDAR2A Antibody [NBP2-19551]

Immunohistochemistry-Paraffin: NMDAR2A Antibody [NBP2-19551]

Immunohistochemistry-Paraffin: NMDAR2A Antibody [NBP2-19551] - NMDAR2A antibody detects GRIN2A protein at membrane on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. NMDAR2A antibody dilution: 1:500.
Western Blot: NMDAR2A Antibody [NBP2-19551]

Western Blot: NMDAR2A Antibody [NBP2-19551]

Western Blot: NMDAR2A Antibody [NBP2-19551] - Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE gel, diluted at 1:1000.
NMDAR2A Antibody

Western Blot: NMDAR2A Antibody [NBP2-19551] -

Western Blot: NMDAR2A Antibody [NBP2-19551] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NMDAR2A antibody (NBP2-19551) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NMDAR2A Antibody

Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody [NBP2-19551] -

Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody [NBP2-19551] - NMDAR2A antibody detects NMDAR2A protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR2A stained by NMDAR2A antibody (NBP2-19551) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.
NMDAR2A Antibody - BSA Free

Western Blot: NMDAR2A Antibody [NBP2-19551] -

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NMDAR2A antibody (NBP2-19551) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NMDAR2A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody [NBP2-19551] -

NMDAR2A antibody detects NMDAR2A protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR2A protein stained by NMDAR2A antibody (NBP2-19551) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for NMDAR2A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 3 using NBP2-19551 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NMDAR2A

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

GluN2A, glutamate receptor, ionotropic, N-methyl D-aspartate 2A, hNR2A, NMDA receptor subtype 2A, N-methyl D-aspartate receptor subtype 2A, N-methyl-D-aspartate receptor subunit 2A, subunit epsilon-1

Gene Symbol

GRIN2A

UniProt

Q12879 (Human)

Additional NMDAR2A Products

Product Documents for NMDAR2A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NMDAR2A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for NMDAR2A Antibody - BSA Free

Customer Reviews for NMDAR2A Antibody - BSA Free (1)

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  • NMDAR2A Antibody
    Name: Janosch Heller
    Application: Immunocytochemistry
    Sample Tested: rat cortical culture (3 weeks old)
    Species: Rat
    Verified Customer | Posted 06/27/2017
    Mixed rat cortical cultures (21 DIV) were labelled with anti-NMDAR2A (1:200) and anti-rabbit Alexa647 (1:500). The antibody labels small punctae along the neuronal processes. No distinct features such as spines are visible.
    Fixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT Blocking Solution & Duration: 3% BSA in PBS with 0.2% saponin, 30 minutes RT Primary Antibody Diluent and Dilutions Tested: 1:200 in 1% BSA in PBS with 0.2% saponin, over night 4°C Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-rabbit Alexa647, 1:500 in PBS with 0.2% saponin, 1 hour, RT
    NMDAR2A Antibody - BSA Free NBP2-19551

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