NMDAR2B Antibody (S59) - BSA Free
Novus Biologicals | Catalog # NBP1-47611
![Western Blot: NMDAR2B Antibody (S59) [NBP1-47611]](https://resources.rndsystems.com/images/products/NMDAR2B-Antibody-S59-Western-Blot-NBP1-47611-img0008.jpg "Western Blot: NMDAR2B Antibody (S59) [NBP1-47611]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # S59
Format
BSA Free
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Product Specifications
Immunogen
Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B
Localization
Cell Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane
Specificity
Detects approx 166kDa. No cross-reactivity against NR2A.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for NMDAR2B Antibody (S59) - BSA Free
Western Blot: NMDAR2B Antibody (S59) [NBP1-47611]
Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NMDAR2B protein using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.![Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611]](https://resources.rndsystems.com/images/products/NMDAR2B-Antibody-S59-Immunocytochemistry-Immunofluorescence-NBP1-47611-img0010.jpg "Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611]")
Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611]
Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2B/NMDAR2B Antibody (D) Composite.![Immunohistochemistry: NMDAR2B Antibody (S59) [NBP1-47611]](https://resources.rndsystems.com/images/products/NMDAR2B-Antibody-S59-Immunohistochemistry-NBP1-47611-img0009.jpg "Immunohistochemistry: NMDAR2B Antibody (S59) [NBP1-47611]")
Immunohistochemistry: NMDAR2B Antibody (S59) [NBP1-47611]
Immunohistochemistry: NMDAR2B Antibody (S59) [NBP1-47611] - Immunohistochemistry analysis using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.![Western Blot: NMDAR2B Antibody (S59) [NBP1-47611]](https://resources.rndsystems.com/images/products/NMDAR2B-Antibody-S59-Western-Blot-NBP1-47611-img0006.jpg "Western Blot: NMDAR2B Antibody (S59) [NBP1-47611]")
Western Blot: NMDAR2B Antibody (S59) [NBP1-47611]
Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - analysis of Human HEK-T lysates showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.![Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611]](https://resources.rndsystems.com/images/products/NMDAR2B-Antibody-S59-Immunocytochemistry-Immunofluorescence-NBP1-47611-img0005.jpg "Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611]")
Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611]
Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.Applications for NMDAR2B Antibody (S59) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:1000
Immunohistochemistry-Paraffin
0.1-1.0 ug/ml
Western Blot
1:1000
Application Notes
1 ug/ml of GluN2B/NR2B Antibody was sufficient for detection of NR2B in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NMDAR2B
Long Name
Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B
Alternate Names
glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, hNR3, MGC142180, NMDA R, NR2B Subunit, NMDAR2B, NMDAR2BMGC142178, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3
Gene Symbol
GRIN2B
UniProt
Additional NMDAR2B Products
Product Documents for NMDAR2B Antibody (S59) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NMDAR2B Antibody (S59) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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