NMDAR2B [p Tyr1252] Antibody - Azide Free
Novus Biologicals | Catalog # NB300-292
![Western Blot: NMDAR2B [p Tyr1252] Antibody [NB300-292]](https://resources.rndsystems.com/images/products/NMDAR2B-[p-Tyr1252]-Antibody-Western-Blot-NB300-292-img0002.jpg "Western Blot: NMDAR2B [p Tyr1252] Antibody [NB300-292]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse, Rat
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide Free
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Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1252 conjugated to KLH. Accession # Q00960
Modification
p Tyr1252
Localization
Integral membrane protein.
Specificity
Specific for endogenous levels of the ~180 kDa NMDAR2B protein phosphorylated at Tyr1252. Immunolabeling is completely eliminated by treatment with lambda-phosphatase. May also show slight reactivity with Tyr1246 of NR2A.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NMDAR2B [p Tyr1252] Antibody - Azide Free
Western Blot: NMDAR2B [p Tyr1252] Antibody [NB300-292]
Western Blot: NMDAR2B [p Tyr1252] Antibody [NB300-292] - Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit phosphorylated at Tyr1252 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1252 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.![Immunohistochemistry: NMDAR2B [p Tyr1252] Antibody [NB300-292]](https://resources.rndsystems.com/images/products/NMDAR2B-[p-Tyr1252]-Antibody-Immunohistochemistry-NB300-292-img0003.jpg "Immunohistochemistry: NMDAR2B [p Tyr1252] Antibody [NB300-292]")
Immunohistochemistry: NMDAR2B [p Tyr1252] Antibody [NB300-292]
Immunohistochemistry: NMDAR2B [p Tyr1252] Antibody [NB300-292] - Immunostaining of 14 DIV rat cortical neurons showing NR2B phosphorylated at Tyr1252 in red and PSD95 in green. Photo courtesy of Gang LiuApplications for NMDAR2B [p Tyr1252] Antibody - Azide Free
Application
Recommended Usage
Immunohistochemistry
1:400
Western Blot
1:1000
Application Notes
This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band 180 kDa.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Format
Azide Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NMDAR2B
Alternate Names
glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, hNR3, MGC142180, NMDAR2BMGC142178, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3
Gene Symbol
GRIN2B
UniProt
Additional NMDAR2B Products
Product Documents for NMDAR2B [p Tyr1252] Antibody - Azide Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NMDAR2B [p Tyr1252] Antibody - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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