NNT Antibody

Novus Biologicals | Catalog # NBP1-32109

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%), Xenopus (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NNT Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NNT. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (88%), Xenopus laevis (93%).

Localization

Mitochondrion inner membrane, Multi-pass membrane protein, Matrix side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NNT Antibody

Western Blot: NNT Antibody [NBP1-32109]

Western Blot: NNT Antibody [NBP1-32109]

Western Blot: NNT Antibody [NBP1-32109] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109]

Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109]

Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Mouse kidney. NNT antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: NNT Antibody [NBP1-32109]

Western Blot: NNT Antibody [NBP1-32109]

Western Blot: NNT Antibody [NBP1-32109] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109]

Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109]

Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - SW480 xenograft, using NNT antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109]

Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109]

Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Mouse stomach. NNT antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
NNT Antibody

Immunocytochemistry/ Immunofluorescence: NNT Antibody [NBP1-32109] -

Immunocytochemistry/ Immunofluorescence: NNT Antibody [NBP1-32109] - NNT antibody detects NNT protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: NNT stained by NNT antibody (NBP1-32109) diluted at 1:500.
NNT Antibody

Western Blot: NNT Antibody [NBP1-32109] -

Western Blot: NNT Antibody [NBP1-32109] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for NNT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NNT

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene.

Alternate Names

MGC126502, mitochondrial, nicotinamide nucleotide transhydrogenaseMGC126503, Pyridine nucleotide transhydrogenase

Gene Symbol

NNT

Additional NNT Products

Product Documents for NNT Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NNT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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