NOP58 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35415

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NOP58 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 360-529 of human NOP58 (NP_057018.1).

Sequence:
RMLAAKTVLAIRYDAFGEDSSSAMGVENRAKLEARLRTLEDRGIRKISGTGKALAKTEKYEHKSEVKTYDPSGDSTLPTCSKKRKIEQVDKEDEITEKKAKKAKIKVKVEEEEEEKVAEEEETSVKKKKKRGKKKHIKEEPLSEEEPCTSTAIASPEKKKKKKKKRENED

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NOP58 Antibody - BSA Free

NOP58 Antibody

Western Blot: NOP58 Antibody [NBP3-35415] -

Western Blot: NOP58 Antibody [NBP3-35415] - Western blot analysis of various lysates using NOP58 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
NOP58 Antibody

Immunocytochemistry/ Immunofluorescence: NOP58 Antibody [NBP3-35415] -

Immunocytochemistry/ Immunofluorescence: NOP58 Antibody [NBP3-35415] - Confocal immunofluorescence analysis of U2OS cells using NOP58 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for NOP58 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NOP58

Nop5p (a.k.a. Nop58p), is localized in the nucleolus and required for pre-18S rRNA processing in Saccharomyces cerevisiae (baker's yeast).

Alternate Names

HSPC120, NOL5, NOP5/NOP58, NOP58 ribonucleoprotein homolog (yeast), NOP5nucleolar protein 58, Nucleolar protein 5, nucleolar protein NOP5/NOP58

Gene Symbol

NOP58

Additional NOP58 Products

Product Documents for NOP58 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NOP58 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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