NPM1 Antibody (FC82291)

Novus Biologicals | Catalog # NB600-1030

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Donkey, Hamster, Monkey

Cited:

Human

Applications

Validated:

Western Blot, Immunoblotting, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # FC82291
View all NPM1 Primary Antibodies »

Product Specifications

Immunogen

rat B23 (nucleophosmin, NPM, numatrin). UniProt accession no. P06748.

Epitope

The epitope recognized by the antibody lies within the 68 amino acids at the C terminus of NPM1.

Reactivity Notes

kangaroo rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Specificity

Detects both the phosphorylated and the unphosphorylated B23 molecule.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NPM1 Antibody (FC82291)

Western Blot: NPM1 Antibody (FC82291) [NB600-1030]

Western Blot: NPM1 Antibody (FC82291) [NB600-1030]

Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse
Immunofluorescence: NPM1 Antibody (FC82291) [NB600-1030]

Immunofluorescence: NPM1 Antibody (FC82291) [NB600-1030]

Immunofluorescence: NPM1 Antibody (FC82291) [NB600-1030] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting: NPM1 Antibody (FC82291) [NB600-1030]

Immunoblotting: NPM1 Antibody (FC82291) [NB600-1030]

Immunoblotting: NPM1 Antibody (FC82291) [NB600-1030] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-B23 Clone: FC82291 Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. RAT2 6. CHO 7. MDBK 8. MDCK

Applications for NPM1 Antibody (FC82291)

Application
Recommended Usage

Immunoblotting

0.5ug/ml

Western Blot

2-4 ug/ml
Application Notes
WB: Detects a band of approximately 37 kDa. Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4) and 1.0% BSA

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NPM1

B23 (also known as NPM, nucleophosmin, numatrin or NO38) is a phosphoprotein, widely expressed in all cell types, and localized in granular regions of the nucleolus associated with pre-ribosomal particles. This protein was found to be involved in several nuclear functions such as assembly and/or intranuclear transport of preribosomal particles, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity and centrosome duplication. Phosphorylation of B23 is mediated by a cyclin complex, CDK2/cyclin E. This phosphorylation causes the dissociation of B23 from the centrosome and enables the duplication of the centrosome during cell mitosis. B23 was also found to be involved in pro-myelocytic leukemia in which the gene for B23 fuses with retinoic acid receptor alpha (RAR alpha). As a consequence, a fusion protein (60-70 kDa) is produced containing parts of the two genes. Monoclonal antibody reacting specifically with B23 is a useful tool for the study of the involvement of B23 in nuclear functions, cytoplasmic/nuclear trafficking, and centrosome duplication.

Long Name

Nucleophosmin/Nucleolar Phosphoprotein B23

Alternate Names

B23, Nucleophosmin, Numatrin

Entrez Gene IDs

4869 (Human); 18148 (Mouse); 25498 (Rat)

Gene Symbol

NPM1

Additional NPM1 Products

Product Documents for NPM1 Antibody (FC82291)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NPM1 Antibody (FC82291)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Acute Myeloid Leukemia
p53 Pathway

Citations for NPM1 Antibody (FC82291)

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Protocols

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