NPM1 Antibody (PD00-91)

Western Blot: NPM1 Antibody (PD00-91) [NBP3-32669]

Western Blot: NPM1 Antibody (PD00-91) [NBP3-32669] - Western blot analysis of NPM1 on different lysates with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/1,000 dilution.

Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A431 cell lysate
Lane 4: C2C12 cell lysate
Lane 4: RAW264.7 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 33 kDa
Observed band size: 38 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32669) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: NPM1 Antibody (PD00-91) [NBP3-32669]

Immunohistochemistry: NPM1 Antibody (PD00-91) [NBP3-32669] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32669) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: NPM1 Antibody (PD00-91) [NBP3-32669]

Immunocytochemistry/ Immunofluorescence: NPM1 Antibody (PD00-91) [NBP3-32669] - Immunocytochemistry analysis of LOVO cells labeling NPM1 with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.