![Western Blot: NQO-1 Antibody (13A1) [NBP2-80395]](https://resources.rndsystems.com/images/products/NQO-1-Antibody-13A1-Western-Blot-NBP2-80395-img0002.jpg "Western Blot: NQO-1 Antibody (13A1) [NBP2-80395]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 13A1
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Product Specifications
Immunogen
Recombinant protein within Human NQO-1 aa 1-285. (SwissProt: P15559 Human; SwissProt: P05982 Rat)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NQO-1 Antibody (13A1)
Western Blot: NQO-1 Antibody (13A1) [NBP2-80395]
Western Blot: NQO-1 Antibody (13A1) [NBP2-80395] - Analysis of NQO-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: SiHa cell lysate, untreated Lane 2: A549 cell lysate, untreated![Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395]](https://resources.rndsystems.com/images/products/NQO-1-Antibody-13A1-Immunohistochemistry-Paraffin-NBP2-80395-img0003.jpg "Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395]")
Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395]
Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395] - Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-NQO-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m![Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395]](https://resources.rndsystems.com/images/products/NQO-1-Antibody-13A1-Immunohistochemistry-Paraffin-NBP2-80395-img0001.jpg "Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395]")
Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395]
Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395] - Analysis in human kidney tissue using NQO-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed withApplications for NQO-1 Antibody (13A1)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:100
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH7.4), 0.2% BSA, 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NQO-1
Long Name
NAD(P)H Dehydrogenase, Quinone 1
Alternate Names
Azoreductase, DHQU, DIA4, Diaphorase-4, Dioxin-inducible 1, DTD, Menadione Reductase, NMOR1, NQO1, QR1, Quinone Reductase 1
Gene Symbol
NQO1
Additional NQO-1 Products
Product Documents for NQO-1 Antibody (13A1)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NQO-1 Antibody (13A1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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