Nrf2 [p Ser40] Antibody (SU0334)

Western Blot: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] - Western blot analysis of Nrf2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb

Flow Cytometry: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] -

Flow Cytometry: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] -Analysis of RIP3 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] -

Immunohistochemistry-Paraffin: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] -Rat kidney tissue using anti-RIP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.