Nucleostemin Antibody (2C8D5) - BSA Free

Novus Biologicals | Catalog # NBP2-37410

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2C8D5

Format

BSA Free
View all Nucleostemin Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Nucleostemin (AA: 1-226) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nucleostemin Antibody (2C8D5) - BSA Free

Western Blot: Nucleostemin Antibody (2C8D5)BSA Free [NBP2-37410]

Western Blot: Nucleostemin Antibody (2C8D5)BSA Free [NBP2-37410]

Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mouse mAb against NIH3T3 (1) and PC-3 (2) cell lysate.
Flow Cytometry: Nucleostemin Antibody (2C8D5) - BSA Free [NBP2-37410]

Flow Cytometry: Nucleostemin Antibody (2C8D5) - BSA Free [NBP2-37410]

Flow Cytometry: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Flow cytometric analysis of Jurkat cells using GNL3 mouse mAb (green) and negative control (purple).
Western Blot: Nucleostemin Antibody (2C8D5)BSA Free [NBP2-37410]

Western Blot: Nucleostemin Antibody (2C8D5)BSA Free [NBP2-37410]

Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mAb against human GNL3 recombinant protein. (Expected MW is 51.9 kDa)
Western Blot: Nucleostemin Antibody (2C8D5)BSA Free [NBP2-37410]

Western Blot: Nucleostemin Antibody (2C8D5)BSA Free [NBP2-37410]

Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mAb against HEK293 (1) and GNL3 (AA: 1-226)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: Nucleostemin Antibody (2C8D5) - BSA Free [NBP2-37410]

ELISA: Nucleostemin Antibody (2C8D5) - BSA Free [NBP2-37410]

ELISA: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Nucleostemin Antibody (2C8D5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nucleostemin

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells.

Alternate Names

GNL3

Gene Symbol

GNL3

Additional Nucleostemin Products

Product Documents for Nucleostemin Antibody (2C8D5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nucleostemin Antibody (2C8D5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Mesenchymal Stem Cell Markers

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Protocols

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FAQs

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