Nucleostemin Antibody

Novus Biologicals | Catalog # NB100-1569

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Nucleostemin Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 500 and the C-terminus (residue 549) of human Guanine Nucleotide Binding Protein-Like 3 (Nucleostemin) using the numbering given in entry NP_055181.3 (GeneID 26354).

Marker

Embryonic Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nucleostemin Antibody

Western Blot: Nucleostemin Antibody [NB100-1569]

Western Blot: Nucleostemin Antibody [NB100-1569]

Western Blot: Nucleostemin Antibody [NB100-1569] - Detection of mouse GNL3 by western blot. Samples:Whole cell lysate (50 ug) from TMCK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody NB100-1569 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: Nucleostemin Antibody [NB100-1569]

Immunohistochemistry: Nucleostemin Antibody [NB100-1569]

Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (NB100-1569). Detection: DAB
Western Blot: Nucleostemin Antibody [NB100-1569]

Western Blot: Nucleostemin Antibody [NB100-1569]

Western Blot: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody NB100-1569 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: Nucleostemin Antibody [NB100-1569]

Immunohistochemistry-Paraffin: Nucleostemin Antibody [NB100-1569]

Immunohistochemistry-Paraffin: Nucleostemin Antibody [NB100-1569] - Human skin carcinoma. Antibody used at a dilution of 1:1,000.
Immunohistochemistry: Nucleostemin Antibody [NB100-1569]

Immunohistochemistry: Nucleostemin Antibody [NB100-1569]

Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-GNL3 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry: Nucleostemin Antibody [NB100-1569]

Immunohistochemistry: Nucleostemin Antibody [NB100-1569]

Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Detection of mouse GNL3 by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (NB100-1569). Detection: DAB
Immunoprecipitation: Nucleostemin Antibody [NB100-1569]

Immunoprecipitation: Nucleostemin Antibody [NB100-1569]

Immunoprecipitation: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GNL3 antibody NB100-1569(lot NB100-1569-3) used for IP at 6 ug per reaction. GNL3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1569-2). For blotting immunoprecipitated GNL3, NB100-1569 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for Nucleostemin Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Nucleostemin

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells.

Alternate Names

GNL3

Entrez Gene IDs

26354 (Human); 30877 (Mouse)

Gene Symbol

GNL3

UniProt

Q9BVP2 (Human)

Additional Nucleostemin Products

Product Documents for Nucleostemin Antibody

Certificate of Analysis

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Product Specific Notices for Nucleostemin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Mesenchymal Stem Cell Markers

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Protocols

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FAQs

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