NUDT11 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-06300
![Western Blot: NUDT11 Antibody [NBP3-06300]](https://resources.rndsystems.com/images/products/NUDT11-Antibody-Western-Blot-NBP3-06300-img0001.jpg "Western Blot: NUDT11 Antibody [NBP3-06300]")
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human NUDT11 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for NUDT11 Antibody - BSA Free
Western Blot: NUDT11 Antibody [NBP3-06300]
Western Blot: NUDT11 Antibody [NBP3-06300] - Anti-NUDT11 rabbit polyclonal antibody at 1:500 dilutionLane A: RT4 Whole Cell LysateLane B: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:18 kDa(We are unsure as to the identity of these extra bands.)
Immunocytochemistry/Immunofluorescence: NUDT11 Antibody [NBP3-06300] -
Immunocytochemistry/Immunofluorescence: NUDT11 Antibody [NBP3-06300] - Immunofluorescence staining of NUDT11 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06300 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.Applications for NUDT11 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NUDT11
Alternate Names
APS1DIPP3B, ASP1, Diadenosine 5'-5'''-P1, diphosphoinositol polyphosphate phosphohydrolase 3, beta, diphosphoinositol polyphosphate phosphohydrolase 3-beta, DIPP3b, DIPP3beta, DIPP-3-beta, DIPP3-beta, EC 3.6.1.-, EC 3.6.1.52, FLJ10628, hDIPP3betahAps1, Nucleoside diphosphate-linked moiety X motif 11, nudix (nucleoside diphosphate linked moiety X)-type motif 11, Nudix motif 11, P6-hexaphosphate hydrolase 3-beta
Gene Symbol
NUDT11
Additional NUDT11 Products
Product Documents for NUDT11 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NUDT11 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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