NUP62 Antibody (M436)

Name: NUP62 Antibody (M436)
Brand: Novus Biologicals
SKU: NBP3-23226
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23226

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # M436

View all NUP62 Primary Antibodies »

Product Specifications

Immunogen

Clone M436 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human nucleoporin p62. This sequence has high homology to similar regions in rat, mouse, and chicken NUP62. [UniProt# P37198]

Specificity

This antibody detects a 62 kDa protein corresponding to the apparent molecular mass of NUP62 on SDS-PAGE immunoblots of human HeLa and rat A7r5 cells. In immunocytochemistry, anti-Nucleoporin p62 specifically stains nuclei and nuclear envelope in paraformaldehyde fixed and NP-40 permeabilized cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for NUP62 Antibody (M436)

Western Blot: NUP62 Antibody (M436) [NBP3-23226] -

Western Blot: NUP62 Antibody (M436) [NBP3-23226] - Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediatefilament protein (lane 1), anti-Nucleoporin p62 NBP3-23226 (lane 2), anti-Hsp60 mitochondrial protein (lane 3), and anti-Calnexin endoplasmic reticulum protein (lane 4).

Immunocytochemistry/Immunofluorescence: NUP62 Antibody (M436) [NBP3-23226] -

Immunocytochemistry/Immunofluorescence: NUP62 Antibody (M436) [NBP3-23226] - Immunocytochemical labeling of Nucleoporin p62 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with mouse monoclonal anti-Nucleoporin p62 (N-terminal region) and the antibody was detected using Goat anti-Mouse secondary antibodies conjugated to DyLight(R) 488.

Applications for NUP62 Antibody (M436)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: NUP62

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq]

Alternate Names

DKFZp547L134,62 kDa nucleoporin, FLJ20822, FLJ43869, IBSNp62, MGC841, nuclear pore glycoprotein p62, nucleoporin 62kD, nucleoporin 62kDa, SNDINucleoporin Nup62

Gene Symbol

NUP62

Additional NUP62 Products

Product Documents for NUP62 Antibody (M436)

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Certificate of Analysis

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Product Specific Notices for NUP62 Antibody (M436)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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