Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Nurr1/NGFI-B beta/NR4A2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide comprising residues 513-526 [TERHGLKEPKRVEE] of the human Nurr1 protein. Based on 100% sequence homology, this antibody is also expected to cross react with rat and mouse Nurr1.

Reactivity Notes

Human and Rat. Other species not yet tested. Mouse reactivity reported in scientific literature (PMID: 18784308).

Specificity

This is specific to the Nurr1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Nurr1/NGFI-B beta/NR4A2 Antibody - BSA Free (NB110-40415) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-Nurr1/NGFI-B beta/NR4A2 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Nurr1/NGFI-B beta/NR4A2 Antibody - BSA Free

Western Blot: Nurr1/NGFI-B beta/NR4A2 AntibodyBSA Free [NB110-40415]

Western Blot: Nurr1/NGFI-B beta/NR4A2 AntibodyBSA Free [NB110-40415]

Western Blot: Nurr1 Antibody [NB110-40415] - Western blot detection of Nurr1 in 20 ug of human hippocampus tissue lysate (lanes 2 and 4) with Nurr1 polyclonal at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. MW marker lane 1. Peptide absorption control lane 3.
Immunohistochemistry: Nurr1/NGFI-B beta/NR4A2 Antibody - BSA Free [NB110-40415]

Immunohistochemistry: Nurr1/NGFI-B beta/NR4A2 Antibody - BSA Free [NB110-40415]

Immunohistochemistry: Nurr1 Antibody [NB110-40415] - Nurr1 staining with Nurr1 polyclonal antibody at a 1:100 dilution in adult rat brain hippocampus. Tissue was fixed in paraformaldehyde and cut into 20 micron sections.

Applications for Nurr1/NGFI-B beta/NR4A2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:200

Western Blot

1:500- 1:2000
Application Notes
This antibody is useful for Western Blot and Immunohistochemistry. Use in Immunoprecipitation reported in scientific literature (PMID 18784308)

Reviewed Applications

Read 1 review rated 5 using NB110-40415 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nurr1/NGFI-B beta/NR4A2

The Nurr1 gene encodes a member of the steroid-thyroid hormone- retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson's. Nurr1 is a stem cell marker and cooperates with PITX3 to promote terminal maturation of murine and human embryonic stem cell cultures to a midbrain dopamine neuron phenotype. In addition, Nurr1 is involved in the regulation of corticotropin-releasing hormone (CRH), which may be linked to and associated with rheumatoid arthritis.

Long Name

Nur-related Factor 1/Nerve Growth Factor Induced B beta/Nuclear Receptor Subfamily 4, Group A, 2

Alternate Names

NGFI-B beta, NOT, NR4A2, TINUR

Entrez Gene IDs

4929 (Human); 54278 (Rat)

Gene Symbol

NR4A2

Additional Nurr1/NGFI-B beta/NR4A2 Products

Product Documents for Nurr1/NGFI-B beta/NR4A2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nurr1/NGFI-B beta/NR4A2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nurr1/NGFI-B beta/NR4A2 Antibody - BSA Free

Customer Reviews for Nurr1/NGFI-B beta/NR4A2 Antibody - BSA Free (1)

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  • Nurr1/NGFI-B beta/NR4A2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: joints
    Species: Mouse
    Verified Customer | Posted 05/23/2018
    antigen retrieval working concentration: 4ug/mL

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