Occludin Antibody (1G7) - Azide and BSA Free

Name: Occludin Antibody (1G7) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00004950-M01
Price: 529 USD
Availability: InStock

Novus Biologicals | Catalog # H00004950-M01

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Citations (3)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 1G7

Format

Azide and BSA Free

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Product Specifications

Immunogen

OCLN (NP_002529, 423 a.a. ~ 522 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT

Marker

Tight Junctions Marker

Specificity

OCLN - occludin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Occludin Antibody (1G7) - Azide and BSA Free

Western Blot: Occludin Antibody (1G7) [H00004950-M01]

Western Blot: Occludin Antibody (1G7) [H00004950-M01] - OCLN monoclonal antibody (M01), clone 1G7 Analysis of OCLN expression in HL-60.

Immunocytochemistry/ Immunofluorescence: Occludin Antibody (1G7) [H00004950-M01]

Immunocytochemistry/Immunofluorescence: Occludin Antibody (1G7) [H00004950-M01] - Analysis of monoclonal antibody to OCLN on HeLa cell. Antibody concentration 10 ug/ml.

Immunoprecipitation: Occludin Antibody (1G7) [H00004950-M01]

Immunoprecipitation: Occludin Antibody (1G7) [H00004950-M01] - Analysis of OCLN transfected lysate using anti-OCLN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with OCLN MaxPab rabbit polyclonal antibody.

ELISA: Occludin Antibody (1G7) [H00004950-M01]

ELISA: Occludin Antibody (1G7) [H00004950-M01] - Detection limit for recombinant GST tagged OCLN is approximately 0.1ng/ml as a capture antibody.

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Applications for Occludin Antibody (1G7) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:500

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Occludin

This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described.

Alternate Names

BLCPMG, OCLN

Entrez Gene IDs

4950 (Human)

Gene Symbol

OCLN

OMIM

602876 (Human)

UniProt

NP_002529 (Human)

Additional Occludin Products

Product Documents for Occludin Antibody (1G7) - Azide and BSA Free

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Product Specific Notices for Occludin Antibody (1G7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.