Occludin Antibody (5A7) - Azide and BSA Free

Novus Biologicals | Catalog # H00004950-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Porcine

Cited:

Rat

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5A7

Format

Azide and BSA Free
View all Occludin Primary Antibodies »

Product Specifications

Immunogen

OCLN (NP_002529, 423 a.a. ~ 522 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33460804)

Marker

Tight Junctions Marker

Specificity

This product is specific for Human OCLN monoclonal antibody (M02), clone 5A7 [Gene ID: 4950].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Occludin Antibody (5A7) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02]

Immunocytochemistry/ Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02]

Immunocytochemistry/Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02] - Occludin labeled Pig SC cells visualized by Alexa 488 conjugated secondary antibody. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02]

Immunocytochemistry/ Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02]

Immunocytochemistry/Immunofluorescence: Occludin Antibody (5A7) [H00004950-M02] - Analysis of monoclonal antibody to OCLN on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: Occludin Antibody (5A7) [H00004950-M02]

ELISA: Occludin Antibody (5A7) [H00004950-M02]

ELISA: Occludin Antibody (5A7) [H00004950-M02] - Detection limit for recombinant GST tagged OCLN is approximately 1ng/ml as a capture antibody.

Applications for Occludin Antibody (5A7) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using H00004950-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Occludin

The Occludin gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.

Alternate Names

BLCPMG, OCLN

Gene Symbol

OCLN

UniProt

NP_002529 (Human)

Additional Occludin Products

Product Documents for Occludin Antibody (5A7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Occludin Antibody (5A7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Occludin Antibody (5A7) - Azide and BSA Free

Customer Reviews for Occludin Antibody (5A7) - Azide and BSA Free (1)

5 out of 5
1 Customer Rating
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Customer Images

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  • Occludin Antibody (5A7)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Pig SC cells
    Species: Pig
    Verified Customer | Posted 06/07/2017
    Mouse monoclonal occludin antibody 5A7 labeling Pig SC cells with Alexa 488
    Occludin Antibody (5A7) - Azide and BSA Free H00004950-M02

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Protocols

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FAQs

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