OCT4 Antibody (7E7) - BSA Free

Novus Biologicals | Catalog # NBP1-51664

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7E7

Format

BSA Free
View all OCT4 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide derived from an internal portion of human OCT4. [Uniprot: Q01860]

Localization

Nucleus

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse OCT4 Antibody (7E7) - BSA Free (NBP1-51664) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for OCT4 Antibody (7E7) - BSA Free

Western Blot: OCT4 Antibody (7E7) [NBP1-51664]

Western Blot: OCT4 Antibody (7E7) [NBP1-51664]

Western Blot: OCT4 Antibody (7E7) [NBP1-51664] - Analysis using OCT4 mouse mAb against NTERA-2 cell lysate.
Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (7E7) [NBP1-51664]

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (7E7) [NBP1-51664]

Immunocytochemistry/Immunofluorescence: OCT4 Antibody (7E7) [NBP1-51664] - Analysis of NTERA-2 cells using OCT4 mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
Flow Cytometry: OCT4 Antibody (7E7) [NBP1-51664]

Flow Cytometry: OCT4 Antibody (7E7) [NBP1-51664]

Flow Cytometry: OCT4 Antibody (7E7) [NBP1-51664] - Flow cytometric analysis of Jurket cells using OCT4 mouse mAb (green) and negative control (purple).
Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (7E7) [NBP1-51664]

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (7E7) [NBP1-51664]

Immunocytochemistry/Immunofluorescence: OCT4 Antibody (7E7) [NBP1-51664] - Analysis of NTERA-2 cells using OCT4 mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).

Applications for OCT4 Antibody (7E7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OCT4

The OCT4 gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. (provided by RefSeq).Tissue specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Alternate Names

class 5, transcription factor 1, MGC22487, Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing DNA-binding protein

Entrez Gene IDs

5460 (Human)

Gene Symbol

POU5F1

UniProt

Q01860

Additional OCT4 Products

Product Documents for OCT4 Antibody (7E7) - BSA Free

Certificate of Analysis

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Product Specific Notices for OCT4 Antibody (7E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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