OCT4 Antibody (OTI9B7)

Western Blot: OCT4 Antibody (OTI9B7) [NBP1-47923]

Western Blot: OCT4 Antibody (OTI9B7) [NBP1-47923] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4.

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (OTI9B7) [NBP1-47923]

OCT4-Antibody-OTI9B7-Immunocytochemistry-Immunofluorescence-NBP1-47923-img0008.jpg

Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923]

Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of paraffin-embedded Human endometrium tissue using anti-41186 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923]

Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of paraffin-embedded Human lung tissue using anti-41186 mouse monoclonal antibody.

Immunohistochemistry: OCT4 Antibody (OTI9B7) [NBP1-47923] -

Immunohistochemistry: OCT4 Antibody (OTI9B7) [NBP1-47923] - Representative DAB IHC-stained sections of MDBMSCC demonstrating nuclear expression of EMA of cells within the tumor nests [(A), brown]. Expression of SOX2 was seen in cells within the tumor nests [(B), brown, thick arrows] & the stroma [(B), brown, arrowheads], & on the endothelium of the microvessels within the stroma [(B), brown, thin arrows]. Expression of SALL4 was limited to cells within the tumor nests [(C), brown, thick arrows] & the endothelium of the microvessels [(C), brown, thin arrows]. OCT4 was also expressed in cells within tumor nests [(D), brown, thick arrows] & the stroma [(D), brown, arrowheads], & the endothelium of the microvessels within the stroma [(D), brown, thin arrows]. Expression of pSTAT3 was detected on cells within the tumor nests [(E), brown, thick arrows] & the stroma [(E), brown, arrowheads], & the endothelium of the microvessels within the stroma [(E), brown, thin arrows]. NANOG was also seen in cells within the tumor nests [(F), brown, thick arrows] & the stroma [(F), brown, arrowheads], & the endothelium of the microvessels within the stroma [(F), brown, thin arrows]. CD44 expression was seen as membranous staining of the tumor nest cells [(G), brown]. Original magnification: 400×. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fsurg.2016.00046/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (OTI9B7) [NBP1-47923] -

Representative IF IHC-stained sections of MDBMSCC demonstrating the expression of pSTAT3 [(A), red] and EMA [(A), green] by cells within the tumor nests. There was a CSC subpopulation remonstrating nuclear co-expression of STAT3 [(B), red] and CD34 [(B), green], appearing as orange, on the endothelium of the microvessels within the stroma; and another subpopulation staining only positively for pSTAT3 within the stroma [(B), red]. Nuclear expression of NANOG [(C), red] was demonstrated on the endothelium of the microvessels which expressed CD34 [(C), green] within stroma. The NANOG+ cells [(C), red] that do not express CD34 were seen within the tumor nests and the stroma. SOX2 [(D), red] was also expressed by cells within tumor nests and the stroma, and the endothelium of the microvessels expressing CD34 [(D), green]. Nuclear expression of both SOX2 [(E), red] and SALL4 [(E), green], appearing as orange, was seen on the cells within the tumor nests and the stroma. Expression of both SOX2 [(F), red] and OCT4 [(F), green], appearing as orange, was seen on cells within the tumor nests and the stroma, and the endothelium of the microvessels within the stroma. pSTAT3 [(G), red] and membranous staining CD44 [(G), green] were co-expressed by cells within the tumor nests. Scale bars: 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27532037), licensed under a CC-BY license. Not internally tested by Novus Biologicals.