ODF2 Antibody (1A1) - Azide and BSA Free

Novus Biologicals | Catalog # H00004957-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, C. elegans

Cited:

Human, Mouse, Nematode - Caenorhabditis elegans

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A1

Format

Azide and BSA Free
View all ODF2 Primary Antibodies »

Product Specifications

Immunogen

ODF2 (NP_002531.3, 706 a.a. ~ 804 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KEHALSKERAAQNKILDLETQLSRTKTELSQLRRSRDDADRRYQSRLQDLKDRLEQSESTNRSMQNYVQFLKSSYANVFGDGPYSTFLTSSPIRSRSPP

Reactivity Notes

C. elegans reactivity reported in scientific literature (PMID: 24231678).

Specificity

ODF2 - outer dense fiber of sperm tails 2 (1A1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ODF2 Antibody (1A1) - Azide and BSA Free

Western Blot: ODF2 Antibody (1A1) [H00004957-M01]

Western Blot: ODF2 Antibody (1A1) [H00004957-M01]

Western Blot: ODF2 Antibody (1A1) [H00004957-M01] - Detection against Immunogen (36.63 KDa).
Immunocytochemistry/ Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01]

Immunocytochemistry/ Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01]

ODF2-Antibody-1A1-Immunocytochemistry-Immunofluorescence-H00004957-M01-img0007.jpg
Immunocytochemistry/ Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01]

Immunocytochemistry/ Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01]

Immunocytochemistry/Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01] - Co-staining of ODF2 (Cy3), DAPI and r-tubulin (Alexa Fluor 488) in RPE1 cell line. ODF2 stains the subdistal appendage of mother centriole. Cells were fixed with cold methanol, 10min. Blocked with 3% BSA for 30min at room temperature. ODF2 antibody was used at 1:100. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01]

Immunocytochemistry/ Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01]

Immunocytochemistry/Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01] - Analysis of monoclonal antibody to ODF2 on HeLa cell. Antibody concentration 10 ug/ml.
ODF2 Antibody (1A1)

Immunocytochemistry/ Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01] -

Immunocytochemistry/ Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01] - The localization & function of DYF-19 are highly conservedEither endogenous (a) or overexpressed (b) FBF1, the mammalian homolog of worm DYF-19, localizes specifically on one centriole with a ring-like pattern in IMCD3 cells. c, FBF1 localizes at the ciliary base, above the basal body. d, In IMCD3 cells, FBF1 localizes above rootlet & subdistal appendage protein ODF2 & completely colocalizes with distal appendage protein CEP164. e, Immuno-EM demonstrates that FBF1 localizes specifically to distal appendages of mother centrioles. f–h, Knock-down of FBF1 leads to severely truncated cilia in most RNAi-treated hTERT-RPE cells. Data are represented as mean of 3 independent experiments (n=200) & error bars indicate s.d. Significant differences were identified by the Student’s t-test. *P<0.001. i & j, The IFT-B component IFT88, but not the IFT-A component IFT140, enters the truncated cilia of FBF1-knockdown hTERT-RPE cells. Arrows indicate the tips of truncated cilia. k, Endogenous IFT54 immunoprecipitates with FBF1 in hTERT-RPE cells. l, HEK293 cells were transiently transfected with FLAG-HA-tagged FBF1, & 48 hours later, cells were subjected to immunoprecipitation using normal mouse IgG (mIgG) or anti-IFT54 antibody. 50 μg protein were loaded into each lane. Bars: c, 1μm; e, 200 nm; others, 20 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24231678), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ODF2 Antibody (1A1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. ICC/IF usage reported in scientific literature. IHC usage reported in scientific literature (PMID: 24231678).

Reviewed Applications

Read 2 reviews rated 5 using H00004957-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ODF2

The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of this gene; however, not all isoforms and variants have been fully described.

Alternate Names

Cenexin, FLJ44866, MGC111096, MGC9034, ODF2/1, ODF2/2, ODF8484-kD, outer dense fiber of sperm tails 2, outer dense fiber protein 2, sperm tail structural protein

Entrez Gene IDs

4957 (Human)

Gene Symbol

ODF2

UniProt

NP_002531 (Human)

Additional ODF2 Products

Product Documents for ODF2 Antibody (1A1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ODF2 Antibody (1A1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ODF2 Antibody (1A1) - Azide and BSA Free

Customer Reviews for ODF2 Antibody (1A1) - Azide and BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • ODF2 Antibody (1A1)
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: RPE1 cells and hTERT RPE-1 cell line from ATCC
    Species: Human
    Verified Customer | Posted 04/09/2019
    Co-staining of ODF2 (Cy3), DAPI and r-tubulin (Alexa Fluor 488) in RPE1 cell line. Cells were fixed with cold methanol. ODF2 antibody was used at 1:100. ODF2 stains the subdistal appendage of mother centriole.
    Fix by cold MEOH for 10 mins. Block with 3% BSA for 0.5 hours at room temperature. Dilute the ab at 1:100 in 3% BSA and incubate with cells for 2 hours at room temperature.
    ODF2 Antibody (1A1) - Azide and BSA Free H00004957-M01
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: hTERT RPE-1 cell line from ATCC
    Species: Human
    Verified Customer | Posted 08/25/2010

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