Olig1 Antibody (24GB5005)

Novus Biologicals | Catalog # NBP3-50442

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB5005
View all Olig1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Olig1 (Uniprot: Q8TAK6)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Olig1 Antibody (24GB5005)

Olig1 Antibody (24GB5005)

Immunocytochemistry/ Immunofluorescence: Olig1 Antibody (24GB5005)[NBP3-50442] -

Immunocytochemical staining of HepG2 cells with Olig1 antibody(1:1,000). Nuclei were stained blue with DAPI; Olig1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Olig1 Antibody (24GB5005)

Flow Cytometry: Olig1 Antibody (24GB5005)[NBP3-50442] -

Flow cytometric analysis of Olig1expression in HepG2 cells using Olig1 antibody (1:2,000). Green, isotype control; red, OLIG1.
Olig1 Antibody (24GB5005)

Western Blot: Olig1 Antibody (24GB5005)[NBP3-50442] -

Western blotting analysis using Olig1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit (oligodendrocyte transcription factor 1.

Applications for Olig1 Antibody (24GB5005)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Olig1

Olig genes have been identified as the earliest known markers of oligodendrocyte lineage determination to date (Zhou et al., 2000). Olig1 is a transcription factor which promotes formation and maturation of oligodendrocytes, especially within the brain. It is expressed in the ventral spinal cord as early as 9.5 dpc and by 15.5 dpc, olig1 is dispersed throughout the gray matter. In the postnatal brain, it is present preferentially in the white matter, such as corpus callosum and cerebellar medulla. Olig1 has been demonstrated as necessary in the repair of brain lesions in patients with multiple sclerosis (Arnett et al. 2004).

Long Name

Oligodendrocyte Lineage Gene 1

Alternate Names

BHLHB6

Gene Symbol

OLIG1

Additional Olig1 Products

Product Documents for Olig1 Antibody (24GB5005)

Certificate of Analysis

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Product Specific Notices for Olig1 Antibody (24GB5005)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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