Olig2 Antibody

Novus Biologicals | Catalog # NBP1-30650

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Immunogen

A synthetic peptide from the n-terminal region of human Olig2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Marker

Oligodendrocyte Marker

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Olig2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:300-1:2000

Immunohistochemistry-Frozen

1:300-1:2000

Western Blot

1:300-1:2000
Application Notes
The optimal dilution should be determined by the end user. Use in IHC-FR reported in scientific literature (PMID 24692237).

Reviewed Applications

Read 1 review rated 3 using NBP1-30650 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Olig2

Olig2 (oligodendrocyte transcription factor 2) is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. It is an antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.

Long Name

Oligodendrocyte Lineage Transcription Factor 2

Alternate Names

BHLHB1, PRKCBP2, RACK17

Entrez Gene IDs

10215 (Human)

Gene Symbol

OLIG2

Additional Olig2 Products

Product Documents for Olig2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Olig2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Olig2 Antibody (1)

3 out of 5
1 Customer Rating
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2 Stars
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Customer Images

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  • Ok antibody for WB
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Colon tissue
    Species: Mouse
    Verified Customer | Posted 10/02/2024
    Dual pink protein marker
    10 mg loading 1:1000 dilution HRP detection To retry with lower concentration
    Olig2 Antibody NBP1-30650

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Olig2 Antibody

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  • Q: I am interested in two of your antibodies: OLIG2 (NBP1-30650) and OLIG2 (NBP1-46676), but I could not find information in your webpage regarding the use of either of the two antibodies in cryosection of mouse tissue. Do you have information about it?, because I would like to use them in spinal cord cryosection from mouse.

    A: We have tested these antibodies in IHC-Fr and IHC- Paraffin, and they are both specific to mouse.

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