Oligodendrocyte Marker O1 Alexa Fluor® 647-conjugated Antibody

FAB1327R Datasheet / COA / SDS

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FAB1327R-100UG

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Oligodendrocyte Marker O1 Alexa Fluor® 647-conjugated Antibody in Flow Cytometry

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Oligodendrocyte Marker O1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat, Chicken

Specificity

Detects human, mouse, rat and chicken Oligodendrocyte Marker O1.

Source

Monoclonal Mouse IgM Clone # O1

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Immunogen

Bovine brain corpus callosum white matter

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of Oligodendrocyte Marker O1 in Rat Differentiated Cortical Stem Cells by Flow Cytometry. Rat differentiated cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB1327R, filled histogram) or mouse IgM isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitution default not known

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oligodendrocyte Marker O1

Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O1 recognizes a glycolipid antigen that is expressed on the surface of late oligodendrocyte progenitors. It has been commonly used in conjunction with Oligodendrocyte Marker O4 antibody to define immature oligodendrocyte (1-6). Progenitors that are O4 antigen-positive and O1 antigen-negative have been shown to differentiate into O1 antigen-positive oligodendrocytes in vitro (7).

References