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Alexa Fluor® 647‑conjugated Antibody

Oligodendrocyte Marker O1
Alexa Fluor® 647‑conjugated Antibody

FAB1327R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1327R-100UG
Detection of Oligodendrocyte Marker O1 in Rat Differentiated Cortical Stem Cells by Flow Cytometry.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Oligodendrocyte Marker O1
Alexa Fluor® 647‑conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat, Chicken
Specificity
Detects human, mouse, rat and chicken Oligodendrocyte Marker O1.
Source
Monoclonal Mouse IgM Clone # O1
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Bovine brain corpus callosum white matter
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Oligodendrocyte Marker O1 in Rat Differentiated Cortical Stem Cells by Flow Cytometry. View Larger

Detection of Oligodendrocyte Marker O1 in Rat Differentiated Cortical Stem Cells by Flow Cytometry. Rat differentiated cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB1327R, filled histogram) or mouse IgM isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitution default not known
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oligodendrocyte Marker O1

Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O1 recognizes a glycolipid antigen that is expressed on the surface of late oligodendrocyte progenitors. It has been commonly used in conjunction with Oligodendrocyte Marker O4 antibody to define immature oligodendrocyte (1-6). Progenitors that are O4 antigen-positive and O1 antigen-negative have been shown to differentiate into O1 antigen-positive oligodendrocytes in vitro (7).

References
  1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
  2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
  3. Sontheimer, H. et al. (1989) Neuron 2:1135.
  4. Hardy, R.J. and V.L. Friedrich Jr. (1996) Development 122:2059.
  5. Reynolds, R. and R. Hardy (1997) J. Neurosci. Res. 47:455.
  6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
  7. Cai, Z. et al. (2001) Brain Res. 898:126.
Alternate Names
Oligodendrocyte Marker O1

Product Datasheets

Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

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Staining Reagents

Flow Cytometry Mouse Lyse Buffer (10X)

FC003
5 Citations
1 Review
Flow

Flow Cytometry Staining Buffer (1X)

FC001
15 Citations
4 Reviews
Flow

Flow Cytometry Human Lyse Buffer (10X)

FC002
1 Citations
1 Review
Flow

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Alexa Fluor® 647‑conjugated Antibody

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Alexa Fluor® 647‑conjugated Antibody and earn rewards!

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