Oligodendrocyte Marker O4 NL637 MAb (Clone O4)

Detection of Mouse O4 by Immunocytochemistry/Immunofluorescence BM-NSCs OLGs differentiation in vitro and transplantation in Shi mice. (A) BM-NSCs were cultured in oligodendrocyte differentiation media for 48 h, 1 week, and 2 weeks for O4 (differentiation marker) and Ki-67 (proliferation marker) staining. (B) Fluorescence staining of BM-NSCs (mCherry+ cells), after 35 days post injection, show myelination (MBP+) in the contralateral striatum. Scale bar = 50 μm. (C) Quantification of tremor of Shi mice treated with OPCs. (D) Transplanted OPCs prolong the survival of Shi mice compared with PBS-treated (n = 9). Symbols represent mean ± SD. ∗p < 0.05, Student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31231194), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse O4 by Immunocytochemistry/Immunofluorescence Fundamental steps of BM-NSCs OLGs differentiation. (A) Expression of Ki-67 at day 38, as shown by immunofluorescence analysis. (B,C) Immunofluorescence staining of progenitor cells at day 38, expressing A2B5, and NG2. (D,E) RIP and O4 staining show cells ramified morphology. (F) Expression of Ki-67 which in late stage of OLGs differentiation (day 52). (G) At the end of differentiation stage, CNPase+ OLGs show typical ramified morphology. (H) OPCs and OLGs proliferation (A,F) was quantified as percentage of Ki67-positive cells. Scale bar = 50 μm. (I) The percentages of A2B5+, NG2+, RIP+, O4+, and CNPase+ cells are present in EOLG+LOLG-DM cultures as compared to OPC-PM cultures. Data are expressed as the mean ± SD, three independent experiments, ∗∗p < 0.01, Student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31231194), licensed under a CC-BY license. Not internally tested by R&D Systems.