OPA3 Antibody (OTI3G4) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-73147
![Western Blot: OPA3 Antibody (OTI3G4)Azide and BSA Free [NBP2-73147]](https://resources.rndsystems.com/images/products/OPA3-Antibody-OTI3G4-Azide-and-BSA-Free-Western-Blot-NBP2-73147-img0001.jpg "Western Blot: OPA3 Antibody (OTI3G4)Azide and BSA Free [NBP2-73147]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3G4
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human OPA3(NP_079412) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for OPA3 Antibody (OTI3G4) - Azide and BSA Free
Western Blot: OPA3 Antibody (OTI3G4)Azide and BSA Free [NBP2-73147]
Western Blot: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OPA3 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiOPA3.(1:2000)![Immunohistochemistry: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147]](https://resources.rndsystems.com/images/products/OPA3-Antibody-OTI3G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73147-img0003.jpg "Immunohistochemistry: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147]")
Immunohistochemistry: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147]
Immunohistochemistry: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-OPA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150).![Immunohistochemistry: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147]](https://resources.rndsystems.com/images/products/OPA3-Antibody-OTI3G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73147-img0002.jpg "Immunohistochemistry: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147]")
Immunohistochemistry: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147]
Immunohistochemistry: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147] - Staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-OPA3 mouse monoclonal antibody. (Heat induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min) (1:150).![Immunohistochemistry: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147]](https://resources.rndsystems.com/images/products/OPA3-Antibody-OTI3G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73147-img0004.jpg "Immunohistochemistry: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147]")
Immunohistochemistry: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147]
Immunohistochemistry: OPA3 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73147] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-OPA3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min)(1:150)Applications for OPA3 Antibody (OTI3G4) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: OPA3
Alternate Names
FLJ22187, FLJ25932, MGA3Optic atrophy 3 (Iraqi-Jewish 'optic atrophy plus'), MGC75494, optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia), optic atrophy 3 protein
Gene Symbol
OPA3
Additional OPA3 Products
Product Documents for OPA3 Antibody (OTI3G4) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for OPA3 Antibody (OTI3G4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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