Optineurin Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00010133-B01P

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
View all Optineurin Primary Antibodies »

Product Specifications

Immunogen

OPTN (NP_001008212.1, 1 a.a. - 577 a.a.) full-length human protein. MSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII

Specificity

OPTN - optineurin,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for Optineurin Antibody - Azide and BSA Free

Western Blot: Optineurin Antibody [H00010133-B01P]

Western Blot: Optineurin Antibody [H00010133-B01P]

Western Blot: Optineurin Antibody [H00010133-B01P] - Analysis of OPTN expression in human placenta.
Immunocytochemistry/ Immunofluorescence: Optineurin Antibody [H00010133-B01P]

Immunocytochemistry/ Immunofluorescence: Optineurin Antibody [H00010133-B01P]

Immunocytochemistry/Immunofluorescence: Optineurin Antibody [H00010133-B01P] - Analysis of purified antibody to OPTN on HeLa cell. Antibody at 10 ug/mL.
Western Blot: Optineurin Antibody [H00010133-B01P]

Western Blot: Optineurin Antibody [H00010133-B01P]

Western Blot: Optineurin Antibody [H00010133-B01P] - Analysis of OPTN expression in transfected 293T cell line by OPTN polyclonal antibody. Lane 1: OPTN transfected lysate (63.47 kDa). Lane 2: Non-transfected lysate.
Simple Western: Optineurin Antibody [H00010133-B01P]

Simple Western: Optineurin Antibody [H00010133-B01P]

Simple Western: Optineurin Antibody [H00010133-B01P] - This antibody (1:25 dilution) was used to probe THP-1 macrophage lysate (concentrations shown) by Simple Western. Simple Western image submitted by a verified customer review.

Applications for Optineurin Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:500
Application Notes
This Optineurin Antibody is validated for Simple Western from a verified customer review.
See Simple Western Antibody Database for Simple Western validation: Tested in THP-1 macrophage lysate 0.2 mg/mL, 0.6 mg/mL and 1.2 mg/mL, separated by Size, antibody dilution of 1:25

Reviewed Applications

Read 1 review rated 4 using H00010133-B01P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Optineurin

This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Alternate Names

ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, OPTN

Entrez Gene IDs

10133 (Human)

Gene Symbol

OPTN

UniProt

NP_001008212.1 (Human)

Additional Optineurin Products

Product Documents for Optineurin Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Optineurin Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

Customer Reviews for Optineurin Antibody - Azide and BSA Free (1)

4 out of 5
1 Customer Rating
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1 Stars
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  • Optineurin Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: THP-1 macrophage lysate
    Species: Human
    Verified Customer | Posted 12/05/2019
    This antibody (1:25 dilution) was used to probe THP-1 macrophage lysate (concentrations shown) by Simple Western.
    Optineurin Antibody - Azide and BSA Free H00010133-B01P

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Protocols

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