![Western Blot: ORAI3 Antibody [NBP2-76956]](https://resources.rndsystems.com/images/products/ORAI3-Antibody-Western-Blot-NBP2-76956-img0001.jpg "Western Blot: ORAI3 Antibody [NBP2-76956]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Synthetic peptide within Human ORAI3 aa 11-60 / 295. (SwissProt: Q9BRQ5 Human; SwissProt: Q6P8G8 Mouse; SwissProt: Q6AXR8 Rat)
Localization
Membrane. Multi-pass membrane protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit ORAI3 Antibody (NBP2-76956) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ORAI3 Antibody
Western Blot: ORAI3 Antibody [NBP2-76956]
Western Blot: ORAI3 Antibody [NBP2-76956] - Analysis of Orai3 using anti-Orai3 antibody at 1/500 dilution.Lane 1 : Raji cell lysatesLane 2 : Raji cell lysates(anti-Orai3 antibody+ immunizing peptide)![Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody [NBP2-76956]](https://resources.rndsystems.com/images/products/ORAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76956-img0008.jpg "Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody [NBP2-76956]")
Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody [NBP2-76956]
Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP2-76956] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Orai3 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956]](https://resources.rndsystems.com/images/products/ORAI3-Antibody-Immunohistochemistry-NBP2-76956-img0009.jpg "Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956]")
Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956]
Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956] - Analysis of human placenta tissue using anti-Orai3 antibody. Counter stained with hematoxylin.![Flow Cytometry: ORAI3 Antibody [NBP2-76956]](https://resources.rndsystems.com/images/products/ORAI3-Antibody-Flow-Cytometry-NBP2-76956-img0010.jpg "Flow Cytometry: ORAI3 Antibody [NBP2-76956]")
Flow Cytometry: ORAI3 Antibody [NBP2-76956]
Flow Cytometry: ORAI3 Antibody [NBP2-76956] - Analysis of A549 cells with Orai3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.![Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody [NBP2-76956]](https://resources.rndsystems.com/images/products/ORAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76956-img0002.jpg "Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody [NBP2-76956]")
Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody [NBP2-76956]
Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP2-76956] - ICC staining Orai3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody [NBP2-76956]](https://resources.rndsystems.com/images/products/ORAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76956-img0003.jpg "Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody [NBP2-76956]")
Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody [NBP2-76956]
Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP2-76956] - ICC staining Orai3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody [NBP2-76956]](https://resources.rndsystems.com/images/products/ORAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76956-img0004.jpg "Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody [NBP2-76956]")
Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody [NBP2-76956]
Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP2-76956] - ICC staining Orai3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956]](https://resources.rndsystems.com/images/products/ORAI3-Antibody-Immunohistochemistry-NBP2-76956-img0005.jpg "Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956]")
Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956]
Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956] - Analysis of rat brain tissue using anti-Orai3 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956]](https://resources.rndsystems.com/images/products/ORAI3-Antibody-Immunohistochemistry-NBP2-76956-img0006.jpg "Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956]")
Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956]
Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956] - Analysis of human liver cancer tissue using anti-Orai3 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956]](https://resources.rndsystems.com/images/products/ORAI3-Antibody-Immunohistochemistry-NBP2-76956-img0007.jpg "Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956]")
Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956]
Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956] - Analysis of mouse testis tissue using anti-Orai3 antibody. Counter stained with hematoxylin.Applications for ORAI3 Antibody
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:1000
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH7.4), 0.2% BSA, 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ORAI3
Long Name
Protein orai-3
Alternate Names
TMEM142C
Gene Symbol
ORAI3
Additional ORAI3 Products
Product Documents for ORAI3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ORAI3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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