Ornithine Decarboxylase Antibody (ODC1/485)

Novus Biologicals | Catalog # NBP2-32887

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # ODC1/485
View all ODC1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Ornithine Decarboxylase protein (Uniprot: P11926)

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34700)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Ornithine Decarboxylase Antibody (ODC1/485)

Western Blot: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Western Blot: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Western Blot: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Analysis using the Azide and BSA Free version of NBP2-32887. Detection of ODC1 in human placental lysate
Immunocytochemistry/ Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Immunocytochemistry/ Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Immunocytochemistry/Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - IF staining of LNCap cells using AF488 labeled ODC1 Monoclonal Antibody (ODC1/485) (GREEN) DAPI was used to stain the cell nucleus (blue). Image using the Azide and BSA Free format of this antibody.
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Ornithine Decarboxylase Antibody (ODC1/485).
Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Flow Cytometric Analysis of human ODC-1 in PC3 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ornithine Decarboxylase antibody (ODC1/485).
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded human prostate carcinoma with ODC-1 Monoclonal Antibody (ODC1/485)
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded human Testicular carcinoma with ODC-1 Monoclonal Antibody (ODC1/485)
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded Rat pancreas stained with ODC1 Monoclonal Antibody (ODC1/485)

Applications for Ornithine Decarboxylase Antibody (ODC1/485)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml

Western Blot

0.5-1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-32887 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ODC1

Ornithine Decarboxylase encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter.

Long Name

Ornithine Decarboxylase 1

Alternate Names

ODC

Gene Symbol

ODC1

UniProt

P11926

Additional ODC1 Products

Product Documents for Ornithine Decarboxylase Antibody (ODC1/485)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ornithine Decarboxylase Antibody (ODC1/485)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ornithine Decarboxylase Antibody (ODC1/485)

Customer Reviews for Ornithine Decarboxylase Antibody (ODC1/485) (1)

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  • Ornithine Decarboxylase Antibody (ODC1/485)
    Name: Andrea Flynn
    Application: Western Blot
    Sample Tested: Human Neuroblastoma whole cell lysate
    Species: Human
    Verified Customer | Posted 07/06/2017
    Used at 1:500 dilution overnight at 4C
    Ornithine Decarboxylase Antibody (ODC1/485) NBP2-32887

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