Ornithine Decarboxylase Antibody (OTI1G6)

Novus Biologicals | Catalog # NBP2-02644

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G6
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Product Specifications

Immunogen

Full length human recombinant protein of human ODC1 (NP_002530) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ornithine Decarboxylase Antibody (OTI1G6)

Western Blot: Ornithine Decarboxylase Antibody (1G6) [NBP2-02644] Analysis of extracts (35ug) from 9 different cell lines by using anti-Ornithine Decarboxylase monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Ornithine Decarboxylase Antibody (OTI1G6) [NBP2-02644]

Immunocytochemistry/ Immunofluorescence: Ornithine Decarboxylase Antibody (OTI1G6) [NBP2-02644]

Immunocytochemistry/Immunofluorescence: Ornithine Decarboxylase Antibody (1G6) [NBP2-02644] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ornithine Decarboxylase.
Flow Cytometry: Ornithine Decarboxylase Antibody (OTI1G6) [NBP2-02644]

Flow Cytometry: Ornithine Decarboxylase Antibody (OTI1G6) [NBP2-02644]

Flow Cytometry: Ornithine Decarboxylase Antibody (1G6) [NBP2-02644] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Ornithine Decarboxylase antibody, and then analyzed by flow cytometry.
Western Blot: Ornithine Decarboxylase Antibody (OTI1G6) [NBP2-02644]

Western Blot: Ornithine Decarboxylase Antibody (OTI1G6) [NBP2-02644]

Western Blot: Ornithine Decarboxylase Antibody (1G6) [NBP2-02644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ornithine Decarboxylase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ornithine Decarboxylase.
Flow Cytometry: Ornithine Decarboxylase Antibody (OTI1G6) [NBP2-02644]

Flow Cytometry: Ornithine Decarboxylase Antibody (OTI1G6) [NBP2-02644]

Flow Cytometry: Ornithine Decarboxylase Antibody (1G6) [NBP2-02644] - Analysis of Jurkat cells, using anti-Ornithine Decarboxylase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for Ornithine Decarboxylase Antibody (OTI1G6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

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Advanced Features

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.57 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ODC1

This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. [provided by RefSeq].

Long Name

Ornithine Decarboxylase 1

Alternate Names

ODC

Entrez Gene IDs

4953 (Human)

Gene Symbol

ODC1

Additional ODC1 Products

Product Documents for Ornithine Decarboxylase Antibody (OTI1G6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Ornithine Decarboxylase Antibody (OTI1G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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