ORP150/HSP12A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32140

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Plant

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ORP150/HSP12A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ORP150/HSP12A. The exact sequence is proprietary.

Reactivity Notes

Bovine (88%).

Localization

Endoplasmic reticulum lumen

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

111 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ORP150/HSP12A Antibody - BSA Free

Western Blot: ORP150/HSP12A Antibody [NBP1-32140]

Western Blot: ORP150/HSP12A Antibody [NBP1-32140]

Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibodydiluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140]

Immunocytochemistry/ Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140]

Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140]

ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP1-32140-img0016.jpg
Western Blot: ORP150/HSP12A Antibody [NBP1-32140]

Western Blot: ORP150/HSP12A Antibody [NBP1-32140]

Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: ORP150/HSP12A Antibody [NBP1-32140]

Western Blot: ORP150/HSP12A Antibody [NBP1-32140]

Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Rat tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140]

Immunocytochemistry/ Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140]

Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Paraffin-embedded CL1-0 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Paraffin-embedded mouse fore brain. ORP150 antibody [C2C3], C-term diluted at 1:500.
ORP150/HSP12A Antibody

Immunocytochemistry/ Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] -

Immunocytochemistry/ Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - Meta-analysis of significant metastatic upregulated proteins in acid exosomes through Prognoscan. a Kaplan-Meyer curves depicting the Overall Survival of metastatic melanoma patients with high (red lines) or low (green lines) expression of the genes listed in Table 1 that resulted statistically significant. Mentel-Cox p-values for each plot are showed. b Western blotting analysis of ctr & pH 6.0 exosomes (20 μg). Cell lysate was used as positive control. Expression of CFL, GSN & HSP90 alpha beta is shown. Ponceau red is shown for equal exosome protein amount. c confocal immunofluorescence analysis with the indicated antibodies on primary (apical & mid region), & metastatic melanoma biopsies from the same patient. Ki67 labeling was used as internal control. Red, CFL, GSN, HYOU1; blue, DAPI; green, Ki67. Bar 50 μm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30290833), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ORP150/HSP12A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] -

ORP150/HSP12A antibody [C2C3], C-term detects ORP150/HSP12A protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ORP150/HSP12A protein stained by ORP150/HSP12A antibody [C2C3], C-term (NBP1-32140) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
ORP150/HSP12A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] -

ORP150/HSP12A antibody [C2C3], C-term detects ORP150/HSP12A protein at cytoplasm by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ORP150/HSP12A protein stained by ORP150/HSP12A antibody [C2C3], C-term (NBP1-32140) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Hoechst 33342 staining.

Applications for ORP150/HSP12A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ORP150/HSP12A

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Long Name

Oxygen Regulated Protein, 150 kDa

Alternate Names

HSP12A, HYOU1

Gene Symbol

HYOU1

Additional ORP150/HSP12A Products

Product Documents for ORP150/HSP12A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ORP150/HSP12A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Heat Shock Proteins (HSPs)

Citations for ORP150/HSP12A Antibody - BSA Free

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Protocols

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