Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free

Novus Biologicals | Catalog # NBP2-37351

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7C10E5

Format

BSA Free
View all Osteoactivin/GPNMB Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Osteoactivin/GPNMB (AA: 31-260) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free (NBP2-37351) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free

Western Blot: Osteoactivin/GPNMB Antibody (7C10E5)BSA Free [NBP2-37351]

Western Blot: Osteoactivin/GPNMB Antibody (7C10E5)BSA Free [NBP2-37351]

Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mouse mAb against PANC1 (1) and PC-3 (2) cell lysate.
Immunohistochemistry: Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free [NBP2-37351]

Immunohistochemistry: Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free [NBP2-37351]

Immunohistochemistry: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using GPNMB mouse mAb with DAB staining.
Western Blot: Osteoactivin/GPNMB Antibody (7C10E5)BSA Free [NBP2-37351]

Western Blot: Osteoactivin/GPNMB Antibody (7C10E5)BSA Free [NBP2-37351]

Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mAb against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)
Western Blot: Osteoactivin/GPNMB Antibody (7C10E5)BSA Free [NBP2-37351]

Western Blot: Osteoactivin/GPNMB Antibody (7C10E5)BSA Free [NBP2-37351]

Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mAb against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free [NBP2-37351]

Immunohistochemistry: Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free [NBP2-37351]

Immunohistochemistry: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPNMB mouse mAb with DAB staining.
ELISA: Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free [NBP2-37351]

ELISA: Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free [NBP2-37351]

ELISA: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteoactivin/GPNMB

The GPNMB gene codes for a type I transmembrane glycoprotein NBM. The GPNMB gene is present in lowly, but not highly, metastic human melanoma cell lines, therefore, it demonstrates involvement in development, proliferation, and differentiation of tissues. Isoform 1 is 572 amino acids long at a mass of nearly 64 kDA and isoform 2 exists at 560 amino acids long at approximately 62 kDA. This gene has been known to interact with various genes such as SMAD4, PLA2G4A, and ITSN2. GPNMB has been researched in its role in retinitis, lung cancer, melanoma, glioblastoma, glaucoma, and breast cancer.

Long Name

Glycoprotein Non-Metastatic Melanoma Protein B

Alternate Names

DC-HIL, GPNMB, HGFIN

Gene Symbol

GPNMB

Additional Osteoactivin/GPNMB Products

Product Documents for Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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