Osteopontin/OPN Antibody (CL10686) - BSA Free

Novus Biologicals | Catalog # NBP3-07980

Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL10686

Format

BSA Free
View all Osteopontin/OPN Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P10451, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Osteopontin/OPN Antibody (CL10686) - BSA Free (NBP3-07980) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Osteopontin/OPN Antibody (CL10686) - BSA Free

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980] - Analysis in human cerebral cortex and tonsil tissues using NBP3-07980 antibody. Corresponding Osteopontin/OPN RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980] - Staining of human cerebellum shows moderate cytoplasmic positivity in microglia.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980] - Staining of human cerebral cortex (Alzheimer disease) shows moderate cytoplasmic positivity in microglia.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980] - Staining of human kidney shows strong cytoplasmic positivity in cells in distal tubules.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Applications for Osteopontin/OPN Antibody (CL10686) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (CL10686) - BSA Free

Certificate of Analysis

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Product Specific Notices for Osteopontin/OPN Antibody (CL10686) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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