Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44052

Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL10686

Format

Azide and BSA Free
View all Osteopontin/OPN Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P10451, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free (NBP3-07980) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free

Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP3-44052]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP3-44052]

Analysis in human cerebral cortex and tonsil tissues using NBP3-44052 antibody. Corresponding Osteopontin/OPN RNA-seq data are presented for the same tissues.
Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP3-44052]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP3-44052]

Staining of human cerebellum shows moderate cytoplasmic positivity in microglia.
Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP3-44052]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP3-44052]

Staining of human cerebral cortex (Alzheimer disease) shows moderate cytoplasmic positivity in microglia.
Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP3-44052]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP3-44052]

Staining of human kidney shows strong cytoplasmic positivity in cells in distal tubules.
Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP3-44052]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP3-44052]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP3-44052]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP3-44052]

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Applications for Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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