Osteopontin/OPN Antibody (OTI5E4)

Novus Biologicals | Catalog # NBP2-45954

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5E4
View all Osteopontin/OPN Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 17-314 of human SPP1(NP_001035147) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Osteopontin/OPN Antibody (OTI5E4)

Western Blot: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Western Blot: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Western Blot: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Osteopontin.
Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Carcinoma of Human prostate tissue using SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (OTI5E4) [NBP2-45954]

Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for Osteopontin/OPN Antibody (OTI5E4)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (OTI5E4)

Certificate of Analysis

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Product Specific Notices for Osteopontin/OPN Antibody (OTI5E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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