OTP Antibody (CL11225) - BSA Free
Novus Biologicals | Catalog # NBP3-15136
Key Product Details
Species Reactivity
Validated:
Human, Rat
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL11225
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q5XKR4, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for OTP Antibody (CL11225) - BSA Free
![Immunocytochemistry/ Immunofluorescence: OTP Antibody (CL11225) [NBP3-15136]](https://resources.rndsystems.com/images/products/OTP-Antibody-CL11222-Immunocytochemistry-Immunofluorescence-NBP3-15136-img0009.jpg "Immunocytochemistry/ Immunofluorescence: OTP Antibody (CL11225) [NBP3-15136]")
Immunocytochemistry/ Immunofluorescence: OTP Antibody (CL11225) [NBP3-15136]
Immunocytochemistry/Immunofluorescence: OTP Antibody (CL11222) [NBP3-15136] - Staining of MCF7 cells using the Anti-OTP monoclonal antibody, showing specific staining in nuclear bodies and in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively.![Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]](https://resources.rndsystems.com/images/products/OTP-Antibody-CL11222-Immunohistochemistry-Paraffin-NBP3-15136-img0008.jpg "Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]")
Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]
Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.![Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]](https://resources.rndsystems.com/images/products/OTP-Antibody-CL11222-Immunohistochemistry-Paraffin-NBP3-15136-img0003.jpg "Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]")
Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]
Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of human hypothalamus shows moderate to strong nuclear positivity in a subset of neurons.![Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]](https://resources.rndsystems.com/images/products/OTP-Antibody-CL11222-Immunohistochemistry-Paraffin-NBP3-15136-img0005.jpg "Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]")
Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]
Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of human lung tumor (typical carcinoid) shows strong nuclear positivity in tumor cells.![Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]](https://resources.rndsystems.com/images/products/OTP-Antibody-CL11222-Immunohistochemistry-Paraffin-NBP3-15136-img0007.jpg "Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]")
Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]
Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of rat hypothalamus shows strong nuclear positivity in a subset of neurons.![Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]](https://resources.rndsystems.com/images/products/OTP-Antibody-CL11222-Immunohistochemistry-Paraffin-NBP3-15136-img0006.jpg "Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]")
Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-15136]
Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of human lymph node shows no positivity in lymphoid cells as expected.Applications for OTP Antibody (CL11225) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: OTP
Alternate Names
homeobox protein orthopedia, MGC3161, orthopedia homeobox, orthopedia homolog, orthopedia homolog (Drosophila)
Gene Symbol
OTP
Additional OTP Products
Product Documents for OTP Antibody (CL11225) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for OTP Antibody (CL11225) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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