Otubain-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83670

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Otubain-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Otubain-1 Antibody - BSA Free

Otubain-1 Antibody - BSA Free Immunohistochemistry-Paraffin: Otubain-1 Antibody - BSA Free [NBP1-83670]

Immunohistochemistry-Paraffin: Otubain-1 Antibody - BSA Free [NBP1-83670]

Staining of human small intestine shows moderate nuclear positivity in glandular cells.
Otubain-1 Antibody - BSA Free Immunohistochemistry-Paraffin: Otubain-1 Antibody - BSA Free [NBP1-83670]

Immunohistochemistry-Paraffin: Otubain-1 Antibody - BSA Free [NBP1-83670]

Staining of human cerebral cortex shows moderate nuclear positivity in glial cells.
Otubain-1 Antibody - BSA Free Immunohistochemistry-Paraffin: Otubain-1 Antibody - BSA Free [NBP1-83670]

Immunohistochemistry-Paraffin: Otubain-1 Antibody - BSA Free [NBP1-83670]

Staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Otubain-1 Antibody - BSA Free Immunohistochemistry-Paraffin: Otubain-1 Antibody - BSA Free [NBP1-83670]

Immunohistochemistry-Paraffin: Otubain-1 Antibody - BSA Free [NBP1-83670]

Staining of human tonsil shows moderate nuclear and cytoplasmic positivity in germinal and non-germinal center cells.
Otubain-1 Antibody - BSA Free Western Blot: Otubain-1 Antibody - BSA Free [NBP1-83670]

Western Blot: Otubain-1 Antibody - BSA Free [NBP1-83670]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Otubain-1 Antibody - BSA Free Western Blot: Otubain-1 Antibody - BSA Free [NBP1-83670]

Western Blot: Otubain-1 Antibody - BSA Free [NBP1-83670]

Analysis in MCF-7 cells) transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-OTUB1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Applications for Otubain-1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Otubain-1

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.

Long Name

OTU Domain, Ubiquitin Aldehyde Binding 1

Alternate Names

OTB1, OTU1, OTUB1, Otubain1

Gene Symbol

OTUB1

Additional Otubain-1 Products

Product Documents for Otubain-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Otubain-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail