Ovalbumin Antibody [Biotin]

Novus Biologicals | Catalog # NB600-924

Novus Biologicals

Key Product Details

Species Reactivity

Mouse, Chicken

Applications

Western Blot, ELISA, Dot Blot

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG
View all Ovalbumin Primary Antibodies »

Product Specifications

Immunogen

Ovalbumin [Hen Egg White] (Uniprot: P01012)

Reactivity Notes

Mouse reactivity reported from a verified customer review. Cross reactivity against Ovalbumin from other sources is unknown.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]

Scientific Data Images for Ovalbumin Antibody [Biotin]

Western Blot: Ovalbumin Antibody [Biotin] [NB600-924] - Lane 1: ~1 ug Ovalbumin protein reduced. Lane 2: 0.25 ug Ovalbumin protein reduced. Lane 3: ~1 ug Ovalbumin protein non-reduced. Lane 4: 0.25 ug Ovalbumin protein non-reduced. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4C. Secondary antibody: Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hr at RT. Block buffer overnight at 4C. Predicted/Observed size: 42.9 kDa, ~36 kDa for ovalbumin.
Western Blot: Ovalbumin Antibody [Biotin] [NB600-924] - Lane 1: Ovalbumin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ovalbumin biotin conjugated primary antibody at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.

Applications for Ovalbumin Antibody [Biotin]

Application
Recommended Usage

ELISA

1:30000

Western Blot

1:500-1:5000
Application Notes
This antibody Biotin Conjugated is suitable for both ELISA, and for western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications that are not stated below.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ovalbumin

Chicken Ovalbumin is the major protein in the "white" of the egg (and a favorite antigen in immunological research). Egg white contains a variety of proteins including ovalbumin, conalbumin, ovomucoid and lysozyme. It belongs to the serpin family and the Ov serpin subfamily. Ovalbumin can cause an allergic reaction in humans. Ovalbumin has been implicated in the development of the egg shell. Immunohistochemistry revealed that ovalbumin is found only in the mammillary bodies of decalcified shell, and is not distributed throughout the shell matrix. These results indicate that ovalbumin is present during the initial phase of shell formation and becomes incorporated into the protein matrix of the mammillary bodies. However, it is not yet clear whether ovalbumin at this site plays a specific role in shell mineralisation.

Alternate Names

Allergen Gal d 2, CAP 2, CAP2, Cytoplasmic antiproteinase 2, Egg albumin, Gal d II, Hen egg white, PI8, Plakalbumin, Protease inhibitor 8, Serpin B8, serpin peptidase inhibitor clade B member 8, SERPINB14, SERPINB8

Gene Symbol

OVAL

UniProt

P01012 (Chicken)

Additional Ovalbumin Products

Product Documents for Ovalbumin Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for Ovalbumin Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ovalbumin Antibody [Biotin]

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