Ovalbumin Antibody - BSA Free

A: Our second Western blot image was from testing using BSA as a blocking solution, so it looks like it doesn't react to BSA at all. This antibody was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Concentration was determined by UV absorbance at 280 nm.

A: We would probably recommend NBP1-05137 as the antibody to use with this as a pair. The reason being we often find it best to run monoclonal and polyclonal antibodies together as the pair in a sandwich ELISA. That way the monoclonal can be used as the capture and the polyclonal as the detection. The reason I would not use the HRP conjugated polyclonal is I believe they are the same antibody just one is conjugated while the other is not. Therefore they would react to the same sites on the protein and compete for binding.

A: We recommend our standard citrate buffer antigen retrieval method or an enzymatic method with alpha-Chymotrypsin. I do not see any special conditions noted by the lab for staining with this one and the fact this is Rabbit on their mouse samples infused with ovalbumin should work well since there won't be any background with the secondary use.

A: We have only validated this antibody for use in IHC-P however it is likely it will also work in IHC-Fr. You may be interested in testing this as we would make you eligble for our Innovators Reward Program. In exchange for a full review of our product with an image, we will issue you a credit for the original purchase price of the antibody.

A: Our second Western blot image was from testing using BSA as a blocking solution, so it looks like it doesn't react to BSA at all. This antibody was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Concentration was determined by UV absorbance at 280 nm.

A: We would probably recommend NBP1-05137 as the antibody to use with this as a pair. The reason being we often find it best to run monoclonal and polyclonal antibodies together as the pair in a sandwich ELISA. That way the monoclonal can be used as the capture and the polyclonal as the detection. The reason I would not use the HRP conjugated polyclonal is I believe they are the same antibody just one is conjugated while the other is not. Therefore they would react to the same sites on the protein and compete for binding.

A: We recommend our standard citrate buffer antigen retrieval method or an enzymatic method with alpha-Chymotrypsin. I do not see any special conditions noted by the lab for staining with this one and the fact this is Rabbit on their mouse samples infused with ovalbumin should work well since there won't be any background with the secondary use.

A: We have only validated this antibody for use in IHC-P however it is likely it will also work in IHC-Fr. You may be interested in testing this as we would make you eligble for our Innovators Reward Program. In exchange for a full review of our product with an image, we will issue you a credit for the original purchase price of the antibody.

A: Our second Western blot image was from testing using BSA as a blocking solution, so it looks like it doesn't react to BSA at all. This antibody was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Concentration was determined by UV absorbance at 280 nm.

A: We would probably recommend NBP1-05137 as the antibody to use with this as a pair. The reason being we often find it best to run monoclonal and polyclonal antibodies together as the pair in a sandwich ELISA. That way the monoclonal can be used as the capture and the polyclonal as the detection. The reason I would not use the HRP conjugated polyclonal is I believe they are the same antibody just one is conjugated while the other is not. Therefore they would react to the same sites on the protein and compete for binding.

A: We recommend our standard citrate buffer antigen retrieval method or an enzymatic method with alpha-Chymotrypsin. I do not see any special conditions noted by the lab for staining with this one and the fact this is Rabbit on their mouse samples infused with ovalbumin should work well since there won't be any background with the secondary use.

A: We have only validated this antibody for use in IHC-P however it is likely it will also work in IHC-Fr. You may be interested in testing this as we would make you eligble for our Innovators Reward Program. In exchange for a full review of our product with an image, we will issue you a credit for the original purchase price of the antibody.

A: Our second Western blot image was from testing using BSA as a blocking solution, so it looks like it doesn't react to BSA at all. This antibody was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Concentration was determined by UV absorbance at 280 nm.

A: We would probably recommend NBP1-05137 as the antibody to use with this as a pair. The reason being we often find it best to run monoclonal and polyclonal antibodies together as the pair in a sandwich ELISA. That way the monoclonal can be used as the capture and the polyclonal as the detection. The reason I would not use the HRP conjugated polyclonal is I believe they are the same antibody just one is conjugated while the other is not. Therefore they would react to the same sites on the protein and compete for binding.

A: We recommend our standard citrate buffer antigen retrieval method or an enzymatic method with alpha-Chymotrypsin. I do not see any special conditions noted by the lab for staining with this one and the fact this is Rabbit on their mouse samples infused with ovalbumin should work well since there won't be any background with the secondary use.

A: We have only validated this antibody for use in IHC-P however it is likely it will also work in IHC-Fr. You may be interested in testing this as we would make you eligble for our Innovators Reward Program. In exchange for a full review of our product with an image, we will issue you a credit for the original purchase price of the antibody.