Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # 182609
View all OX40 Ligand/TNFSF4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse OX40 Ligand/TNFSF4
Gln49-Leu198
Accession # P43488

Specificity

Detects mouse OX40 Ligand/TNFSF4 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) OX40, recombinant human (rh) OX40 Ligand, rmFas Ligand, rmLIGHT, or rhLIGHT is observed. In Western blots, 100% cross-reactivity with rmTNF‑ alpha is observed, 15‑30% with rmTRANCE and rmTRAIL is observed, and no cross-reactivity with rmBAFF, rmEDA, rmGITR Ligand, rmLIGHT, rmTWEAK, rmFas Ligand, rhAPRIL, or rhOX40 Ligand is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for OX40 Ligand/TNFSF4 Antibody (182609) [Biotin]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: OX40 Ligand/TNFSF4

CD252 is a 35 kD member of the TNF superfamily also known as OX40L and CD134L. The OX40 ligand is expressed on activated B cells and antigen presenting cells. OX40 ligand interacts with OX40 antigen (CD134) expressed predominantly on activated T cells to increase proliferation and IL-2 production, and to enhance proliferation and immunoglobulin secretion in activated B cells. The RM134L antibody can inhibit OX40/OX40 ligand interactions and block the costimulatory activity of OX40L.

Alternate Names

CD252, gp34, TNFSF4

Gene Symbol

TNFSF4

Additional OX40 Ligand/TNFSF4 Products

Product Documents for OX40 Ligand/TNFSF4 Antibody (182609) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for OX40 Ligand/TNFSF4 Antibody (182609) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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