OXPAT Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-16013

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 270-463 of human OXPAT (NP_001013728.2). PPESRRRSQAELETLVLSRSLTQELQGTVEALESSVRGLPAGAQEKVAEVRRSVDALQTAFADARCFRDVPAAALAEGRGRVAHAHACVDELLELVVQAVPLPWLVGPFAPILVERPEPLPDLADLVDEVIGGPDPRWAHLDWPAQQRAWEAEHRDGSGNGDGDRMGVAGDICEQEPETPSCPVKHTLMPELDF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OXPAT Antibody - Azide and BSA Free

Western Blot: OXPAT AntibodyAzide and BSA Free [NBP3-16013]

Western Blot: OXPAT AntibodyAzide and BSA Free [NBP3-16013]

Western Blot: OXPAT Antibody [NBP3-16013] - Western blot analysis of extracts of various cell lines, using OXPAT antibody (NBP3-16013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: OXPAT AntibodyAzide and BSA Free [NBP3-16013]

Western Blot: OXPAT AntibodyAzide and BSA Free [NBP3-16013]

Western Blot: OXPAT Antibody [NBP3-16013] - Western blot analysis of extracts of HepG2 cells, using OXPAT antibody (NBP3-16013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
OXPAT Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: OXPAT Antibody - Azide and BSA Free [NBP3-16013] -

Immunocytochemistry/ Immunofluorescence: OXPAT Antibody - Azide and BSA Free [NBP3-16013] - Immunofluorescence analysis of HepG2 cells using OXPAT Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for OXPAT Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: OXPAT

OXPAT is a novel PAT-1 protein that is expressed in highly oxidative tissues. Lipid droplet proteins of the PAT family regulate cellular neutral lipid stores. OXPAT is induced by physiological (fasting), pathophysiological (insulin deficiency), pharmacological (peroxisome proliferator-activated receptor [PPAR] agonists), and genetic (muscle-specific PPAR alpha overexpression) disturbances that increase fatty acid utilization. It is suggested that OXPAT is a marker for PPAR activation and fatty acid oxidation. OXPAT may also contribute to adaptive responses to the fatty acid responsibility that accompanies fasting, insulin deficiency, and overnutrition responses that are defective in obesity and type 2 diabetes.

Alternate Names

lipid storage droplet protein 5, LSDA5, LSDP5, MLDP, OXPAT, perilipin 5, perilipin-5

Gene Symbol

PLIN5

Additional OXPAT Products

Product Documents for OXPAT Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for OXPAT Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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