p130 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82455

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all p130 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IAGSPLTPRRVTEVRADTGGLGRSITSPTTLYDRYSSPPASTTRRRLFVENDSPSDGGTPGRMPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p130 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: p130 Antibody [NBP1-82455]

Immunocytochemistry/ Immunofluorescence: p130 Antibody [NBP1-82455]

Immunocytochemistry/Immunofluorescence: p130 Antibody [NBP1-82455] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: p130 Antibody [NBP1-82455]

Immunohistochemistry-Paraffin: p130 Antibody [NBP1-82455]

Immunohistochemistry-Paraffin: p130 Antibody [NBP1-82455] - Staining of human tonsil shows strong nuclear positivity in lymphoid cells.
Immunohistochemistry-Paraffin: p130 Antibody [NBP1-82455]

Immunohistochemistry-Paraffin: p130 Antibody [NBP1-82455]

Immunohistochemistry-Paraffin: p130 Antibody [NBP1-82455] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in granular and molecular layer.
Immunohistochemistry-Paraffin: p130 Antibody [NBP1-82455]

Immunohistochemistry-Paraffin: p130 Antibody [NBP1-82455]

Immunohistochemistry-Paraffin: p130 Antibody [NBP1-82455] - Staining of human endometrium shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: p130 Antibody [NBP1-82455]

Immunohistochemistry-Paraffin: p130 Antibody [NBP1-82455]

Immunohistochemistry-Paraffin: p130 Antibody [NBP1-82455] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
p130 Antibody

Western Blot: p130 Antibody [NBP1-82455] -

Western Blot: p130 Antibody [NBP1-82455] - Analysis in human cell line MOLT-4.
p130 Antibody - BSA Free Chromatin Immunoprecipitation ChIP: p130 Antibody - BSA Free

Chromatin Immunoprecipitation ChIP: p130 Antibody - BSA Free

ChIP-Exo-Seq composite graph for Anti-p130 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.

Applications for p130 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p130

Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.

Alternate Names

FLJ26459, p130, pRb2, RB2, RBR-2, retinoblastoma-like 2 (p130), retinoblastoma-like protein 2,130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2

Gene Symbol

RBL2

Additional p130 Products

Product Documents for p130 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for p130 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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