p21/CIP1/CDKN1A Antibody (CL13081) - BSA Free

Novus Biologicals | Catalog # NBP3-18554

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13081

Format

BSA Free
View all p21/CIP1/CDKN1A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P38936, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for p21/CIP1/CDKN1A Antibody (CL13081) - BSA Free

Western Blot: p21/CIP1/CDKN1A Antibody (CL13081) [NBP3-18554]

Western Blot: p21/CIP1/CDKN1A Antibody (CL13081) [NBP3-18554]

Western Blot: p21/CIP1/CDKN1A Antibody (CL13081) [NBP3-18554] - Analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (CL13081) [NBP3-18554]

Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (CL13081) [NBP3-18554]

Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (CL13081) [NBP3-18554] - Staining of A549 cells using the Anti-CDKN1A monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).

Applications for p21/CIP1/CDKN1A Antibody (CL13081) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Western Blot

1 ug/ml
Application Notes
For use with ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p21/CIP1/CDKN1A

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Long Name

p21 Cyclin Dependent Kinase 4 Inhibitor 1A

Alternate Names

CDKN1A, CIP1

Gene Symbol

CDKN1A

Additional p21/CIP1/CDKN1A Products

Product Documents for p21/CIP1/CDKN1A Antibody (CL13081) - BSA Free

Certificate of Analysis

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Product Specific Notices for p21/CIP1/CDKN1A Antibody (CL13081) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Cell Cycle

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Protocols

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